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 >  Protein>MASP3 >MA3-H52H3

Human MASP3 (450-721) Protein, His Tag (active enzyme)

分子别名(Synonym)

MASP3, MASP1, CRARF1, RaRF

表达区间及表达系统(Source)

Human MASP3 (450-721), His Tag (MA3-H52H3) is expressed from human 293 cells (HEK293). It contains AA Ile 450 - Val 721 (Accession # P48740-2).

Predicted N-terminus: Ile 450

Request for sequence

蛋白结构(Molecular Characterization)

MASP3 Structure

This protein carries a polyhistidine tag at the C-terminus

The protein has a calculated MW of 31.6 kDa. The protein migrates as 38-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

MASP3 SDS-PAGE

Human MASP3 (450-721), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)

Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Lys-ThioBenzyl ester (Z-Lys-SBzl), in the presence of 5,5’Dithio-bis (2-nitrobenzoic acid) (DTNB).The specific activity is >7500 pmol/min/µg,as measured under the described conditions (QC tested).

Protocol

 

背景(Background)

MASP3 is a member of the MASP family. The MASP family proteins were first
discovered as complexes with mannose-binding lectin (MBL) and therefore named MBL-associated
serine proteases, but later, they were found to interact with ficolins, and later still, collectin-10
and collectin-11. As well as proteolytic enzymes (MASP-1, MASP-2, MASP-3), the group includes
non-enzymatic factors (MAp19, MAp44). In this review, the association-specific factors of the lectin
pathway with haematologic malignancies and related infections are discussed.

 

前沿进展

 
 
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MASP3靶点信息
英文全称:Mannan-binding lectin serine protease 1
中文全称:甘露聚糖结合凝集素相关丝氨酸蛋白酶1
种类:
上市药物数量:0详情
临床药物数量:1详情
最高研发阶段:临床二期
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