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 >  Protein>IL-2 R beta & IL-2 R gamma >ILG-C82F4

Biotinylated Cynomolgus IL-2 R beta&IL-2 R gamma Heterodimer Protein, Fc,Avitag™&Fc,Avitag™ (MALS verified)

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分子别名(Synonym)

IL-2 R beta & IL-2 R gamma,IL-2RB & IL-2RG

表达区间及表达系统(Source)

Biotinylated Cynomolgus IL-2RB&IL-2RG, Fc,Avitag&Fc,Avitag (ILG-C82F4) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Thr 240 (IL-2RB) & Leu 23 - Pro 255 (IL-2RG) (Accession # Q38J85-1 (IL-2RB) & Q38JL2-1 (IL-2RG)).

Predicted N-terminus: Ala 27 (IL-2RB) & Leu 23 (IL-2RG)

Request for sequence

蛋白结构(Molecular Characterization)

IL-2 R beta & IL-2 R gamma Structure

Biotinylated Cynomolgus IL-2RB&IL-2RG, Fc,Avitag&Fc,Avitag is produced by co-expression of IL-2RB andIL-2RG, has a calculated MW of 53.0 kDa (IL-2RB) and 55.4 kDa (IL-2RG). Subunit IL-2RB is fused with a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™) and subunit IL-2RA&IL-2RG is fused with a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™). The reducing (R) protein migrates as 70-85 kDa and 90-110 kDa respectively due to glycosylation.

标记(Labeling)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

IL-2 R beta & IL-2 R gamma SDS-PAGE

Biotinylated Cynomolgus IL-2RB&IL-2RG, Fc,Avitag&Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

IL-2 R beta & IL-2 R gamma SEC-MALS

The purity of Biotinylated Cynomolgus IL-2RB&IL-2RG, Fc,Avitag&Fc,Avitag (Cat. No. ILG-C82F4) is more than 90% and the molecular weight of this protein is around 150-170 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

IL-2 R beta & IL-2 R gamma ELISA

Immobilized Human IL-2, Tag Free at 5 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus IL-2RB&IL-2RG, Fc,Avitag&Fc,Avitag (Cat. No. ILG-C82F4) with a linear range of 0.02-0.625 μg/mL (QC tested).

Protocol

 

活性(Bioactivity)-BLI

IL-2 R beta & IL-2 R gamma BLI

Loaded Biotinylated Cynomolgus IL-2RB&IL-2RG, Fc,Avitag&Fc,Avitag (Cat. No. ILG-C82F4) on SA Biosensor, can bind Human IL-2, Tag Free with an affinity constant of 2.34 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

 

背景(Background)

Both Interleukin-2 receptor subunit beta and Interleukin-2 receptor subunit gamma are receptor for interleukin-2. Common subunit for the receptors for a variety of interleukins. Interacts with SHB upon interleukin stimulation. Probably in association with IL15RA, involved in the stimulation of neutrophil phagocytosis by IL15. This beta subunit is involved in receptor mediated endocytosis and transduces the mitogenic signals of IL2. IL2R exists in 3 different forms: a high affinity dimer, an intermediate affinity monomer (beta subunit), and a low affinity monomer (alpha subunit). The high and intermediate affinity forms also associate with a gamma subunit.

 

前沿进展

 
 
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IL-2 R beta & IL-2 R gamma靶点信息
英文全称:Interleukin-2 receptor beta chain
中文全称:白细胞介素-2受体β链
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:12详情
最高研发阶段:临床三期
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