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 >  Protein>Angiopoietin-like 3 >AN3-H52H3

Human Angiopoietin-like 3 / ANGPTL3 Protein, His Tag

分子别名(Synonym)

ANGPTL3,ANGPT5,ANG-5,Angiopoietin-5,FHBL2

表达区间及表达系统(Source)

Human Angiopoietin-like 3, His Tag (AN3-H52H3) is expressed from human 293 cells (HEK293). It contains AA Ser 17 - Glu 460 (Accession # Q9Y5C1-1).

Predicted N-terminus: Ser 17

Request for sequence

蛋白结构(Molecular Characterization)

Angiopoietin-like 3 Structure

This protein carries a polyhistidine tag at the C-terminus. This protein contains a furin-like convertase cleavage site, 221-RAPR-224, and will be partially processed into N and C-terminal fragment with calculated MW of 24.3 kDa and 29.4 kDa respectively. The protein migrates as 55-70 kDa (full lengh), 31-33 kDa (C-terminal fragment) when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, 0.05% CHAPS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Angiopoietin-like 3 SDS-PAGE

Human Angiopoietin-like 3, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

 

活性(Bioactivity)-ELISA

Angiopoietin-like 3 ELISA

Immobilized Human Angiopoietin-like 3, His Tag (Cat. No. AN3-H52H3) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-Human ANGPTL3 Antibody, Human IgG1 with a linear range of 0.5-2 ng/mL (QC tested).

Protocol

 

背景(Background)

Angiopoietin-like protein 3 (ANGPTL3) is also known as Angiopoietin-related protein 3, Angiopoietin-5 (ANGPT5 / ANG-5), is a member of the angiopoietin-like family of secreted factors. ANGPTL3 / ANGPT5 is predominantly expressed in the liver, and has the characteristic structure of angiopoietins, consisting of a signal peptide, N-terminal coiled-coil domain and the C-terminal fibrinogen (FBN)-like domain. The FBN-like domain in angiopoietin-like 3 protein was shown to bind alpha-5/beta-3 integrins, and this binding induced endothelial cell adhesion and migration. This protein may also play a role in the regulation of angiogenesis. Angptl3 also acts as dual inhibitor of lipoprotein lipase (LPL) and endothelial lipase (EL), and increases plasma triglyceride and HDL cholesterol in rodents. ANGPTL3 inhibit endothelial lipase to catalyze HDL-phospholipid and increase HDL-PL levels. Circulating PL-riched HDL particles have high cholesterol efflux abilities.

 

前沿进展

 
 
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Angiopoietin-like 3靶点信息
英文全称:Angiopoietin like 3
中文全称:血管生成素样蛋白-3
种类:Homo sapiens
上市药物数量:1详情
临床药物数量:9详情
最高研发阶段:批准上市
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