膜杰作
Star Staining
| ID | Components | Size |
| RAS025-C01 | Pre-coated SARS-CoV-2 Spike Protein Microplate | 1 plate |
| RAS025-C02 | SARS-CoV-2 Antibody Positive Control | 100 μL |
| RAS025-C03 | SARS-CoV-2 Antibody Negative Control | 100 μL |
| RAS025-C04 | HRP-Anti-Human IgG | 200 μL |
| RAS025-C05 | 10xWashing Buffer | 50 mL |
| RAS025-C06 | Dilution Buffer | 50 mL |
| RAS025-C07 | Substrate Solution | 12 mL |
| RAS025-C08 | Stop Solution | 7 mL |
背景（Background）
The newly identified Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has posed a serious threat to human health. A rapid and effective Assay kit detecting the levels of anti-SARS-CoV-2 in human serum can facilitate research on characterization of antibodies produced in response to SARS-CoV-2 infection.
应用说明（Application）
This kit is developed for titer measurement of Anti-SARS-CoV-2 Antibody IgG (Spike Trimer) in human serum.
It is for research use only.
重构方法（Reconstitution）
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
存储 & 运输（Storage & Shipping）
1. Unopened kit should be stored at 2℃-8℃ upon receiving.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
原理（Assay Principles）
This assay kit employs a standard indirect-ELISA format, providing a rapid detection of anti-SARS-CoV-2 IgG in serum by SARS-CoV-2 Spike Protein. The kit consists of Pre-coated SARS-CoV-2 Spike Protein Microplate, an Positive Control, an Negative Control,an HRP-Anti-Human IgG secondary antibody and related buffer.
Your experiment will include 4 simple steps:
a) Add your sample to the plate, take the Anti-SARS-CoV-2 antibody as Control sample. The samples and Control sample are diluted by Dilution Buffer.
b) Add a diluted Secondary antibody HRP-Anti-Human IgG to the plate. The Secondary antibody is diluted by Dilution Buffer.
c) Wash the plate and add TMB or other colorimetric HRP substrate.
d) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.
典型数据-Typical Data Please refer to Ds document for the assay protocol.
Detection of Anti-SARS-CoV-2 Antibody, Human IgG1 titer by Indirect-ELISA Assay.
Immobilized SARS-CoV-2 Spike Protein at 2 μg/mL (100 μL/well) can bind monoclonal Anti-SARS-CoV-2 Antibody, Human IgG1 in 1:400 human serum. Detection was performed using HRP- Anti-human IgG antibody with sensitivity of 98 ng/mL (QC tested).
