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Human CD47-coupled Magnetic Beads

热销产品推荐:The Magnetic Stand (Cat.No. MB-01 & Cat.No. MB-02) can be used in conjunction with Beads.
ClinMaxTM细胞因子检测试剂盒

ItemsSize (2mg)Size(5mg X 2)
Particle size2 μm2 μm
Physical appearancePowder mixturePowder mixture
Amount of Coupled Protein≈1302 pmol (22 μg) CD47 protein/mg beads≈1302 pmol (22 μg) CD47 protein/mg beads
Binding Capacity>250 pmol (45 μg) Anti-CD47 antibody/mg beads>250 pmol (45 μg) Anti-CD47 antibody/mg beads
FormulationPBS, pH7.4, with 10% TrehalosePBS, pH7.4, with 10% Trehalose
Reconstitution2 mL sterile deionized water (1 mg beads/mL)5 mL sterile deionized water (1 mg beads/mL)

背景(Background)

The Human CD47-coupled Magnetic Beads is produced by coupling biotinylated CD47 protein to streptavidin-conjugated magnetic beads. Because Streptavidin (SA) has an extraordinarily high affinity for biotin with a dissociation constant (Kd) on the order of 10-14 mol/L, the biotinylated protein can bind to the SA beads irreversibly. The pre-coupled beads are ready to use for capturing anti-CD47 antibody from your sample with high specificity.

表达区间及表达系统(Source)

Human CD47 Protein is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Pro 139(Accession # Q08722).

应用说明(Application)

This product is intended for immunocapture, biopanning and flow cytometry.

*The Isotype control (Cat. No. SMB-B01) is sold separately.

重构方法(Reconstitution)

See Certificate of Analysis (CoA) for detailed instruction.

存储(Storage)

Upon receipt, please store the beads at -20°C for 1 year in lyophilized state.

Please avoid more than 3 freeze-thaw cycles once reconstitution, immediate use after reconstitution is highly recommended.

原理(Assay Principles)

Antibody Purification: 1. Resuspend the lyophilized beads by adding the buffer of choice. 2. Add analyte to the suspension, mix and incubate to enable specific binding of the beads and the target protein. 3. Magnetize beads, remove supernatant, and wash unbound protein fractions to capture target protein-bound beads. 4. Wash, magnetize the beads and collect purified target protein for use in downstream applications.

The magnetic beads technology makes use of the easy and efficient collection of beads in magnetic field to facilitate antibody purification in a simple workflow of “bind-wash-elute”. In contrast to common separation techniques, this method does not require columns or centrifugation, and is therefore ideal in high-throughput applications.

制剂(Formulation)

Please contact us for detailed information.

Contact us for customized product form or formulation.

 

典型数据-Typical Data

CD47 TYPICAL DATA

Immobilized 15.8 μg CD47/1mg beads can bind the Anti CD47 Ab with an EC50 of 0.5720 μg/mL.

Protocol

 

前沿进展

 
 
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CD47靶点信息
英文全称:Cluster of differentiation 47
中文全称:分化群47
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:55详情
最高研发阶段:临床三期
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