登录 | 注册    关注公众号  
微信公众号
搜索
 >  Protein>TACI >TAI-H52H3

Human TACI / TNFRSF13B Protein, His Tag

分子别名(Synonym)

CD267,TACI,TNFRSF13B

表达区间及表达系统(Source)

Human TACI, His Tag (TAI-H52H3) is expressed from human 293 cells (HEK293). It contains AA Ser 2 - Thr 166 (Accession # O14836-1).

Request for sequence

蛋白结构(Molecular Characterization)

TACI Structure

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 20.5 kDa. The protein migrates as 19-22 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, 0.2 M Arginine, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

TACI SDS-PAGE

Human TACI, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

 

活性(Bioactivity)-ELISA

TACI ELISA

Immobilized Human BAFF, Fc Tag, active trimer (Cat. No. BAF-H5261) at 2 μg/mL (100 μL/well) can bind Human TACI, His Tag (Cat. No. TAI-H52H3) with a linear range of 1-16 ng/mL (QC tested).

Protocol

TACI ELISA

Immobilized Human TACI, His Tag (Cat. No. TAI-H52H3) at 2 μg/mL (100 μL/well) can bind Biotinylated Human APRIL, Fc,Avitag (Cat. No. APL-H82F5) with a linear range of 2-39 ng/mL (Routinely tested).

Protocol

TACI ELISA

Immobilized Human TACI, His Tag (Cat. No. TAI-H52H3) at 2 μg/mL (100 μL/well) can bind Human BAFF, Fc Tag, active trimer (Cat. No. BAF-H5261) with a linear range of 0.2-2 ng/mL (Routinely tested).

Protocol

 

背景(Background)

Transmembrane activator and CAML interactor (TACI), also known as tumor necrosis factor receptor superfamily member 13B (TNFRSF13B). It was originally discovered because of its ability to interact with calcium-modulator and cyclophilin ligand (CAML). TACI was later found to play a crucial role in humoral immunity by interacting with two members of the TNF family: BAFF and APRIL.
The present study demonstrated that, in NSCLC, a proliferation-inducing ligand (APRIL), B-cell maturation antigen (BCMA)and transmembrane activator and CAML interactor (TACI) proteins are abnormally expressed by immunohistochemistry, reverse transcription-quantitative polymerase chain reaction and western blotting. In addition, the expression of APRIL, BCMA and TACI were observed to be involved in extracellular signal-regulated kinase (ERK)1/2 activation in A549 cells.

 

前沿进展

 
 
货号/价格
文档
联系电话:
+86 400-682-2521(全国)
010-53681107(北京)
021-50850665(上海)
运输方式
订单邮箱:
order.cn@acrobiosystems.com
技术支持邮箱:
tech.cn@acrobiosystems.com
TACI靶点信息
英文全称:Tumor necrosis factor receptor superfamily member 13B
中文全称:肿瘤坏死因子受体13B基因
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:1详情
最高研发阶段:临床一期
查看更多信息
前沿进展
点击查看详细

消息提示

请输入您的联系方式,再点击提交!

确定