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Biotinylated Mouse SIRP alpha / CD172a Protein, His,Avitag™ (MALS verified)

分子别名(Synonym)

SIRP alpha,SHPS1,SIRPA,CD172A,BIT,SHPS-1,MFR,MYD-1,MYD1,PTPNS1,SIRPalpha,p84,SIRP-alpha,CD172

表达区间及表达系统(Source)

Biotinylated Mouse SIRP alpha, His,Avitag (SIA-M82E2) is expressed from human 293 cells (HEK293). It contains AA Lys 32 - Asn 373 (Accession # P97797-1).

Predicted N-terminus: Lys 32

蛋白结构(Molecular Characterization)

Online(Lys 32 - Asn 373) P97797-1

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 41.5 kDa. The protein migrates as 55-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Biotinylation)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Biotin:Protein Ratio)

The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Biotinylated Mouse SIRP alpha, His,Avitag (Cat. No. SIA-M82E2) SDS-PAGE gel

Biotinylated Mouse SIRP alpha, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS

Biotinylated Mouse SIRP alpha, His,Avitag (Cat. No. SIA-M82E2) MALS images

The purity of Biotinylated Mouse SIRP alpha, His,Avitag (Cat. No. SIA-M82E2) was more than 90% and the molecular weight of this protein is around 63-78kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Measured by its binding ability in a functional ELISA. Immobilized Biotinylated Mouse SIRP alpha, His,Avitag (Cat. No. SIA-M82E2) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Mouse CD47, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CD7-M525a) with a linear range of 0.2-5 ng/mL (QC tested).

Protocol

背景(Background)

Tyrosine-protein phosphatase non-receptor type substrate 1 (SHPS1) is also known as CD172 antigen-like family member A (CD172a), Macrophage fusion receptor, MyD-1 antigen, Signal-regulatory protein alpha (SIRPA or SIRP alpha) or p84, is a member of the SIRP family, and also belongs to the immunoglobulin superfamily. SIRP alpha is Ubiquitous and highly expressed in brain. SIRPA / CD172a is immunoglobulin-like cell surface receptor for CD47 and acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. SIRPA / SHPS-1 supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment and may play a key role in intracellular signaling during synaptogenesis and in synaptic function By similarity. SIRPA / MyD1 involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin and mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.

 

前沿进展

 
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SIRP alpha靶点信息
英文全称:Signal-regulatory protein alpha
中文全称:信号调节蛋白α
种类:Homo sapiens
上市药物数量:1详情
临床药物数量:4详情
最高研发阶段:批准上市
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