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Human Gastrin-71 Protein, Fc tag

分子别名(Synonym)

Gastrin-71,Gastrin,GAST,Gastrin component

表达区间及表达系统(Source)

Human Gastrin-71 Protein, Fc tag (GAN-H5253) is expressed from human 293 cells (HEK293). It contains AA Ser 22 - Phe 92 (Accession # P01350-1).

Predicted N-terminus: Ser 22

蛋白结构(Molecular Characterization)

Online(Ser 22 - Phe 92) P01350-1

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 34.5 kDa. The protein migrates as 33-35 kDa and 40 kDa under reducing (R) condition (SDS-PAGE).

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human Gastrin-71 Protein, Fc tag (Cat. No. GAN-H5253) SDS-PAGE gel

Human Gastrin-71 Protein, Fc tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

背景(Background)

Gastrin is a peptide hormone that stimulates secretion of gastric acid (HCl) by the parietal cells of the stomach and aids in gastric motility. It is released by G cells in the pyloric antrum of the stomach, duodenum, and the pancreas. Gastrin binds to cholecystokinin B receptors to stimulate the release of histamines in enterochromaffin-like cells, and it induces the insertion of K+/H+ ATPase pumps into the apical membrane of parietal cells (which in turn increases H+ release into the stomach cavity). Its release is stimulated by peptides in the lumen of the stomach.

 

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