膜杰作
Star Staining
分子别名（Synonym）
GAS6,GAS-6,AXLLG
表达区间及表达系统（Source）
Human GAS6, His Tag (GA6-H5249) is expressed from human 293 cells (HEK293). It contains AA Asp 279 - Ala 678 (Accession # Q14393-2).
Predicted N-terminus: Asp 279
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 46.0 kDa. The protein migrates as 45-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Human GAS6, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Human GAS6, His Tag (Cat. No. GA6-H5249) is more than 90% and the molecular weight of this protein is around 44-58 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Human GAS6, His Tag (Cat. No. GA6-H5249) at 5 μg/mL (100 μL/well) can bind Human Axl, Fc Tag (Cat. No. AXL-H5253) with a linear range of 0.1-10 ng/mL (QC tested).
Protocol
活性（Bioactivity）-SPR
Human Axl, His Tag (Cat. No. AXL-H5226) immobilized on CM5 Chip can bind Human GAS6, His Tag (Cat. No. GA6-H5249) with an affinity constant of 3.4 nM as determined in SPR assay (Biacore 8K) (Routinely tested).
Protocol
Human MERTK, His Tag (Cat. No. MEK-H52H6) immobilized on CM5 Chip can bind Human GAS6, His Tag (Cat. No. GA6-H5249) with an affinity constant of 50.9 nM as determined in SPR assay (Biacore 8K) (Routinely tested).
Protocol
背景（Background）
GAS6 (Growth arrest-specific protein 6) is also known as AXL receptor tyrosine kinase ligand, AXLLG. Ligand for tyrosine-protein kinase receptors AXL, TYRO3 and MER whose signaling is implicated in cell growth and survival, cell adhesion and cell migration. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses.
