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 >  Protein>CD99 >CD9-HF254

FITC-Labeled Human CD99 / MIC2 Protein, Fc Tag

分子别名(Synonym)

CD99,HBA71,MIC2,MIC2X,MIC2Y,MSK5X,12E7

表达区间及表达系统(Source)

FITC-Labeled Human CD99, Fc Tag (CD9-HF254) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 122 (Accession # P14209-1 ).

Predicted N-terminus: Asp 23

Request for sequence

蛋白结构(Molecular Characterization)

CD99 Structure

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 36.2 kDa. The protein migrates as 45-55 kD under reducing (R) condition (SDS-PAGE) due to glycosylation.

偶联(Conjugate)

FITC

Excitation source: 488 nm spectral line, argon-ion laser

Excitation Wavelength: 488 nm

Emission Wavelength: 535 nm

标记(Labeling)

The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular sieve treatment during purification process.

蛋白标记度(Protein Ratio)

The FITC to protein molar ratio is 1.5-3.5.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

CD99 SDS-PAGE

FITC-Labeled Human CD99, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

 

背景(Background)

CD99 antigen is also known as MIC2, single-chain type-1 glycoprotein, HBA71, MIC2X, MIC2Y, MSK5X, 12E7, which belongs to the CD99 family. CD99 / MIC2 is expressed on all leukocytes but highest on thymocytes. Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. CD99 / MIC2 plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. CD99 acts at the same site as, but independently of PECAM1. There is also experimental evidence that it binds to cyclophilin A.

 

前沿进展

 
 
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CD99靶点信息
英文全称:CD99 antigen
中文全称:CD99抗原
种类:
上市药物数量:0详情
临床药物数量:0详情
最高研发阶段:临床前
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