膜杰作
Star Staining
& Cat.No. | Items | Size （2mg） | Size（5mg X 2） |
| Particle size | 2 μm | 2 μm |
| Physical appearance | Powder mixture | Powder mixture |
| Amount of Coupled Protein | ≈640 pmol (24.2 μg) Human Nectin-4/mg beads | ≈640 pmol (24.2 μg) Human Nectin-4/mg beads |
| Binding Capacity | >280 pmol (20μg) Anti-Nectin-4 Ab/mg beads | >280 pmol (20μg) Anti-Nectin-4 Ab/mg beads |
| Formulation | PBS, pH7.4, with 10% Trehalose | PBS, pH7.4, with 10% Trehalose |
| Reconstitution | 2 mL sterile deionized water (1 mg beads/mL) | 5 mL sterile deionized water (1 mg beads/mL) |
背景（Background）
The Human Nectin-4-coupled Magnetic Beads is produced by coupling biotinylated human Nectin-4 protein to streptavidin-conjugated magnetic beads. The pre-coupled beads are ready to use for capturing anti-Nectin-4 antibody from your sample with high specificity.
表达区间及表达系统（Source）
Human Nectin-4 Protein is expressed from human 293 cells (HEK293). It contains AA Gly 32 - Ser 349(Accession # Q96NY8-1).
应用说明（Application）
This product is intended for immunocapture, biopanning and flow cytometry. This product is produced non-sterile.
重构方法（Reconstitution）
See Certificate of Analysis (CoA) for detailed instruction.
存储（Storage）
Upon receipt, please store the Beads at -20°C. The shelf life is 6 months at -20 °C.
Please avoid more than 3 freeze-thaw cycles once reconstitution, immediate use after reconstitution is highly recommended.
原理（Assay Principles）
Antibody Purification: 1. Reconstitute the beads with ultrapure water to 1 mg/mL, and wash the beads for three times. 2. Dilute the biotinalyted protein or antibodies with Assay Buffer and add to the beads. Incubate for 60 minutes at room temperature in a rotary mixer. 3. Wash the beads for four times, and add Assay Buffer to the beads. The beads are ready to use for the next experiment.
The magnetic beads technology makes use of the easy and efficient collection of beads in magnetic field to facilitate antibody purification in a simple workflow of “bind-wash-elute”. In contrast to common separation techniques, this method does not require columns or centrifugation, and is therefore ideal in high-throughput applications.
制剂（Formulation）
Please contact us for detailed information.
Contact us for customized product form or formulation.
典型数据-Typical Data
Immobilized 24.2 μg Nectin-4 / 1 mg beads can bind Anti-Nectin-4-Ab with an EC50 of 0.5457 μg/mL.
Protocol
Immobilized 24.2 μg Nectin-4 / 1 mg beads can bind Nectin-1 with (Cat. No. PV1-H5253) an EC50 of 0.4625 μg/mL.
Protocol
