膜杰作
Star Staining
分子别名（Synonym）
IL3,MCGF,MGC79398,MGC79399,MULTI-CSF,Interleukin-3
表达区间及表达系统（Source）
Human IL-3 Protein, His Tag (IL3-H52H9) is expressed from human 293 cells (HEK293). It contains AA Ala 20 - Phe 152 (Accession # P08700-1).
Predicted N-terminus: Ala 20
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 17.0 kDa. The protein migrates as 25-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 0.3 EU per μg by the LAL method.
无菌（Sterility）
Negative
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Human IL-3 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS
The purity of Human IL-3 Protein, His Tag (Cat. No. IL3-H52H9) is more than 85% and the molecular weight of this protein is around 20-30 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Human IL-3 Protein, His Tag (Cat. No. IL3-H52H9) at 5 μg/mL (100 μL/well) can bind Human IL-3 R alpha Protein, Llama IgG2b Fc Tag (Cat. No. ILA-H5255) with a linear range of 0.039-2.5 μg/mL (QC tested).
Protocol
活性（Bioactivity）-BLI
Loaded Human IL-3 R alpha, Fc Tag (Cat. No. ILA-H5252) on Protein A Biosensor, can bind Human IL-3 Protein, His Tag (Cat. No. IL3-H52H9) with an affinity constant of 0.71 μM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).
Protocol
Loaded Biotinylated Human IL-3 R alpha, Fc,Avitag (Cat. No. ILA-H82F3) on SA Biosensor, can bind Human IL-3 Protein, His Tag (Cat. No. IL3-H52H9) with an affinity constant of 0.35 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
背景（Background）
Interleukin 3 is also known as IL-3, is a protein that in humans is encoded by the IL3 gene. Interleukin-3 (IL-3) is an interleukin, a type of biological signal (cytokine) that can improve the body's natural response to disease as part of the immune system. It acts by binding to the interleukin-3 receptor. The IL-3 receptor is a heterodimer of the IL-3 specific α-chain and the common β-chain, βc, which is also used by GM-CSF and IL-5. [3] Binding of IL-3 can also involve substitution of the βc by a βIL-3-chain that appears to be specific for IL-3 . Binding of IL-3 to its cognate receptor(s) induces activation of Jak2, phosphorylation of multiple Stats, and the PI3K/Akt pathway. IL-3 may play an important role in the development of airway inflammation associated with asthma. The human IL-3 gene encodes a protein 152 amino acids long, and the naturally occurring IL-3 is glycosylated. The human IL-3 gene is located on chromosome 5, only 9 kilobases from the GM-CSF gene, and its function is quite similar to GM-CSF.
