膜杰作
Star Staining
| ID | Components | Size |
| RAS043-C01 | Pre-coated SARS-CoV-2 Spike Trimer(B.1.617.2) Microplate | 1 plate |
| RAS043-C02 | Positive Control | 100 μL |
| RAS043-C03 | Negative Control | 100 μL |
| RAS043-C04 | HRP-Anti-Human IgG | 200 μL |
| RAS043-C05 | 10xWashing Buffer | 50 mL |
| RAS043-C06 | Dilution Buffer | 50 mL |
| RAS043-C07 | Substrate Solution | 12 mL |
| RAS043-C08 | Stop Solution | 7 mL |
背景（Background）
Multiple variants of SARS-CoV-2 are circulating globally and posting new challenges to human health. The variant B.1.1.7 identified in the United Kingdom (UK) carries a large number of mutations and is reported to spread more easily and quickly than other variants. The variant B.1.351 identified in South Africa ，P.1 identified in Brazil and B.1.617.2 in India show evidence of increased transmissibility and resistance to established immunity. A common mutation identified in the U.K, South African and Brazilian variants is N501Y, and the mutation identified in B.1.617.2 is T478K,which resides on the receptor-binding domain (RBD) of SARS-CoV-2 spike protein. To evaluate the impacts of the new variants, a rapid and effective assay kit detecting the levels of antibody against the mutant is in urgent need.
应用说明（Application）
This Kit is developed for qualitative detection or titer measurement of Anti-SARS-CoV-2 (B.1.617.2) antibody IgG (Spike Trimer) in human serum.
It is for research use only.
存储 & 运输（Storage & Shipping）
1. Unopened kit should be stored at 2℃-8℃ upon receiving.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
原理（Assay Principles）
This assay kit employs a standard indirect-ELISA format, providing a rapid detection of Anti-SARS-CoV-2 antibodies in serum by SARS-CoV-2 Spike RBD. The kit consists of Pre-coated SARS-CoV-2 Spike RBD Microplate, an Positive Control, an Negative Control,an HRP-Anti-Human IgG secondary antibody and related buffer.
Your experiment will include 4 simple steps:
a) Add your sample to the plate. The samples and Control sample are diluted by Dilution Buffer.
b) Add diluted Secondary antibody HRP-Anti-Human IgG to the plate. The Secondary antibody is diluted by Dilution Buffer.
c) Wash the plate and add TMB or other colorimetric HRP substrate.
d) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.
