膜杰作
Star Staining
分子别名（Synonym）
S1 protein NTD,Spike protein S1 NTD,BetaCoV S1-NTD
表达区间及表达系统（Source）
SARS-CoV-2 Spike NTD, His Tag (B.1.1.529/Omicron) (SPD-C522d) is expressed from human 293 cells (HEK293). It contains AA Ser 13 - Leu 303 (Accession # QHD43416.1 (A67V, HV69-70del, T95I, G142D, VYY143-145del, N211del, L212I, ins214EPE)). The spike mutations are identified on the SARS-CoV-2 Omicron variant (Pango lineage: B.1.1.529; GISAID clade: GR/484A; Nextstrain clade: 21K).
Predicted N-terminus: Ser 13
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 34.6 kDa. The protein migrates as 50-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
SARS-CoV-2 Spike NTD, His Tag (B.1.1.529/Omicron) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of SARS-CoV-2 Spike NTD, His Tag (B.1.1.529/Omicron) (Cat. No. SPD-C522d) is more than 90% and the molecular weight of this protein is around 48-64 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized SARS-CoV-2 Spike NTD, His Tag (B.1.1.529/Omicron) (Cat. No. SPD-C522d) at 1 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Spike NTD Antibody, Chimeric mAb (Cat. No. SPD-M121) with a linear range of 0.1-3 ng/mL (QC tested).
Protocol
Immobilized Anti-SARS-CoV-2 Spike NTD Antibody, Chimeric mAb, Human IgG1 (AM121) (Cat. No. SPD-M121) at 1 μg/mL (100 μL/well) can bind SARS-CoV-2 Spike NTD, His Tag (B.1.1.529/Omicron) (Cat. No. SPD-C522d) with a linear range of 0.016-0.125 μg/mL (Routinely tested).
Protocol
背景（Background）
It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
