膜杰作
Star Staining
产品描述（Product Details）
Endo H is a recombinant glycosidase cloned from Streptomyces plicatus and overexpressed in E. coli. It cleaves the chitobiose core of high-mannose oligosaccharides and a limited number of hybrid oligosaccharides from asparagine-linked glycoproteins.
应用说明（Application）
Endo H cleaves within the chitobiose core of high mannose and some hybrid oligosaccharides from N-linked glycoproteins.
单位定义（Unit Definition）
One unit is defined as the amount of enzyme required to remove >95% of the carbohydrate from 10µg of denatured RNase B in 1 hour at 37°C in a total reaction volume of 10µl.
纯度（Purity）
>95% as determined by SDS-PAGE.
酶活（Enzyme Activity）
>500 U/μL
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
制剂（Formulation）
Supplied as 0.2 μm filtered solution in 20 mM Tris, 50 mM NaCl, pH7.5.
Contact us for customized product form or formulation.
运输（Shipping）
This product is supplied and shipped with blue ice, please inquire the shipping cost.
存储（Storage）
This product is stable after storage at:
- The product MUST be stored at -20°C or lower upon receipt.
- -20°C for 6 months under sterile conditions.
活性（Bioactivity）
One unit remove > 95% of the carbohydrate from 10 µg of denatured RNase B in 1 hour at 37°C in a total reaction volume of 10 µl (QC tested).
