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 >  Protein>Glucosylceramidase >GLE-H52H3

Human Glucosylceramidase Protein, His Tag

分子别名(Synonym)

Glucosylceramidase,GBA,Acid beta-glucosidase,Alglucerase,beta-glucocerebrosidase,D-glucosyl-N-acylsphingosine glucohydrolase,EC 3.2.1.45,GBA,GBA1,GC,GCB,GLUC,glucosidase, beta, acid,glucosidase, beta,acid (includes glucosylceramidase),Glucosylceramidase,Imiglucerase,lysosomal glucocerebrosidase

表达区间及表达系统(Source)

Human Glucosylceramidase, His Tag (GLE-H52H3) is expressed from human 293 cells (HEK293). It contains AA Ala 40 - Gln 536 (Accession # P04062-1).

Predicted N-terminus: Ala 40

Request for sequence

蛋白结构(Molecular Characterization)

Glucosylceramidase Structure

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 57.5 kDa. The protein migrates as 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Supplied as 0.2 μm filtered solution in PBS with Glycerol as protectant.

Contact us for customized product form or formulation.

运输(Shipping)

This product is supplied and shipped with dry ice, please inquire the shipping cost.

存储(Storage)

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. The product MUST be stored at -70°C or lower upon receipt;
  2. -70°C for 3 months under sterile conditions.
 

电泳(SDS-PAGE)

Glucosylceramidase SDS-PAGE

Human Glucosylceramidase, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

 

背景(Background)

Glucosylceramidase that catalyzes, within the lysosomal compartment, the hydrolysis of glucosylceramide/GlcCer into free ceramide and glucose. Thereby, it plays a central role in the degradation of complex lipids and the turnover of cellular membranes. Through the production of ceramides,it participates in the PKC-activated salvage pathway of ceramide formation and plays a role in cholesterol metabolism. It may either catalyze the glucosylation of cholesterol, through a transglucosylation reaction that transfers glucose from glucosylceramide to cholesterol. Defects in Glucosylceramidase are the cause of Gaucher disease, also known as glucocerebrosidase deficiency. Gaucher disease is the most prevalent lysosomal storage disease, characterized by accumulation of glucosylceramide in the reticulo-endothelial system. Currently, enzyme replacement therapy is used to treat patients with the disease.

 

前沿进展

 
 
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Glucosylceramidase靶点信息
英文全称:Glucosylceramidase
中文全称:葡糖苷酰鞘氨醇酶
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:2详情
最高研发阶段:临床一期
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