搜索
 >  Kits>Spike RBD >RAS-T057

Anti-SARS-CoV-2 (B.1.1.529) Antibody IgG Titer Serologic Assay Kit (Spike RBD)

For research use only.

IDComponentsSize
RAS057-C01Pre-coated SARS-CoV-2 Spike RBD (B.1.1.529) Microplate1 plate
RAS057-C02Positive Control100 μL
RAS057-C03Negative Control100 μL
RAS057-C04HRP-Anti-Human IgG200 μL
RAS057-C0510xWashing Buffer50 mL
RAS057-C06Dilution Buffer50 mL
RAS057-C07Substrate Solution12 mL
RAS057-C08Stop Solution7 mL

Background/背景

As of Nov 29th, confirmed cases of a new variant of concern (VOC) B.1.1.529 have been reported in South Africa, Botswana and more than 12 other countries. Thanks to the rapid sequencing efforts of South African scientists, the whole world is now alerted about this potentially devastating lineage, which was assigned the name Omicron by WHO.Omicron is a highly divergent variant with a high number of mutations (26-35) in the spike. Identification of some familiar mutations that also appeared on other VOCs gives rises to concerns of its immune escape potential and higher transmissibility. To facilitate the mutant-related research, drug trials and vaccine development, a high-throughput assay to measure IgG antibodies against the mutants is in urgent need.

Application/应用

This Kit is developed for qualitative detection or titer measurement of Anti-SARS-CoV-2 (B.1.1.529) Antibody IgG (Spike RBD) in human serum.

It is for research use only.

Storage/存储

The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

Assay Principles/原理

This assay kit employs a standard indirect-ELISA format, providing a rapid detection of Anti-SARS-CoV-2 antibodies in serum by SARS-CoV-2 Spike RBD. The kit consists of Pre-coated SARS-CoV-2 Spike RBD Microplate, an Positive Control, an Negative Control, an HRP-Anti-Human IgG secondary antibody and related buffer.

Your experiment will include 4 simple steps:

a) Add your sample to the plate. The samples and Control sample are diluted by Dilution Buffer.

b) Add diluted Secondary antibody HRP-Anti-Human IgG to the plate. The Secondary antibody is diluted by Dilution Buffer.

c) Wash the plate and add TMB or other colorimetric HRP substrate.

d) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.

 

Typical Data/典型数据

Biotinylated Human ELISA

For determination of antibody titer: the positive sample was diluted with a gradient, and the antibody titer of the sample corresponds to the highest dilution factor that still yields a positive reading.

Protocol

前沿进展

 
 
货号/价格
开发建议
文档
联系电话:
400-682-2521(全国)
010-57737274(北京)
021-26136428(上海)
运输方式
订单邮箱:
order.cn@acrobiosystems.com
技术支持邮箱:
techsupport@acrobiosystems.com
前沿进展
点击查看详细

消息提示

请输入您的联系方式,再点击提交!

确定