膜杰作
Star Staining
| ID | Components | Size |
| RAS057-C01 | Pre-coated SARS-CoV-2 Spike RBD (B.1.1.529) Microplate | 1 plate |
| RAS057-C02 | SARS-CoV-2 Antibody Positive Control | 100 μL |
| RAS057-C03 | SARS-CoV-2 Antibody Negative Control | 100 μL |
| RAS057-C04 | HRP-Anti-Human IgG | 200 μL |
| RAS057-C05 | 10xWashing Buffer | 50 mL |
| RAS057-C06 | Dilution Buffer | 50 mL |
| RAS057-C07 | Substrate Solution | 12 mL |
| RAS057-C08 | Stop Solution | 7 mL |
背景（Background）
As of Nov 29th, confirmed cases of a new variant of concern (VOC) B.1.1.529 have been reported in South Africa, Botswana and more than 12 other countries. Thanks to the rapid sequencing efforts of South African scientists, the whole world is now alerted about this potentially devastating lineage, which was assigned the name Omicron by WHO.Omicron is a highly divergent variant with a high number of mutations (26-35) in the spike. Identification of some familiar mutations that also appeared on other VOCs gives rises to concerns of its immune escape potential and higher transmissibility. To facilitate the mutant-related research, drug trials and vaccine development, a high-throughput assay to measure IgG antibodies against the mutants is in urgent need.
应用说明（Application）
This Kit is developed for qualitative detection or titer measurement of Anti-SARS-CoV-2 (B.1.1.529) Antibody IgG (Spike RBD) in human serum.
It is for research use only.
存储 & 运输（Storage & Shipping）
1. Unopened kit should be stored at 2℃-8℃ upon receiving.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
原理（Assay Principles）
This assay kit employs a standard indirect-ELISA format, providing a rapid detection of Anti-SARS-CoV-2 antibodies in serum by SARS-CoV-2 Spike RBD. The kit consists of Pre-coated SARS-CoV-2 Spike RBD Microplate, an Positive Control, an Negative Control, an HRP-Anti-Human IgG secondary antibody and related buffer.
Your experiment will include 4 simple steps:
a) Add your sample to the plate. The samples and Control sample are diluted by Dilution Buffer.
b) Add diluted Secondary antibody HRP-Anti-Human IgG to the plate. The Secondary antibody is diluted by Dilution Buffer.
c) Wash the plate and add TMB or other colorimetric HRP substrate.
d) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.
