膜杰作
Star Staining
组分（Materials Provided）
| ID | Components | Size |
| EP115-C01 | Pre-coated Human ACE2 Microplate | 1 plate |
| EP115-C02 | SARS-CoV-2 Inhibitor | 20 μg |
| EP115-C03 | HRP-SARS-CoV-2 Spike RBD(B.1.1.529) | 15 μg |
| EP115-C04 | 10xWashing Buffer | 50 mL |
| EP115-C05 | Dilution Buffer | 50 mL |
| EP115-C06 | Substrate Solution | 12 mL |
| EP115-C07 | Stop Solution | 7 mL |
产品概述（Product Overview）
It's been reported that coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
应用说明（Application）
This kit is developed for screening inhibitors of SARS-CoV-2 (B.1.1.529).
It is for research use only.
重构方法（Reconstitution）
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
存储（Storage）
2-8℃
质量管理控制体系（QMS）
典型数据-Typical Data Please refer to DS document for the assay protocol.
Incubation of SARS-CoV-2 inhibitor:Blocking the binding of HRP-SARS-CoV-2 Spike RBD(B.1.529) to Human ACE2.
Serial dilutions of SARS-CoV-2 inhibitor (Catalog # EP115-C02) (1:1 serial dilutions, from 25ug/mL to 0.049ug/mL(171.9nM to 0.34nM)) and loaded onto the plate coated by human ACE2 in the presence of HRP-SARS-CoV-2 Spike RBD(B.1.1.529). Background was subtracted from data points prior to log transformation and curve fitting.
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