Anti-SARS-CoV-2 (B.1.1.529) Antibody IgG3 Titer Serologic Assay Kit (Spike RBD)

背景（Background）

As of Nov 29th, confirmed cases of a new variant of concern (VOC) B.1.1.529 have been reported in South Africa, Botswana and more than 12 other countries. Thanks to the rapid sequencing efforts of South African scientists, the whole world is now alerted about this potentially devastating lineage, which was assigned the name Omicron by WHO.Omicron is a highly divergent variant with a high number of mutations (26-35) in the spike. Identification of some familiar mutations that also appeared on other VOCs gives rises to concerns of its immune escape potential and higher transmissibility. To facilitate the mutant-related research, drug trials and vaccine development, a high-throughput assay to measure subtype IgG antibodies against the mutants is in urgent need,including IgG1, IgG2 IgG3, IgG4.

应用说明（Application）

This kit is developed for qualitative detection or titer measurement of Anti-SARS-CoV-2 (B.1.1.529) antibody IgG3 (Spike RBD) in human serum.

It is for research use only.

重构方法（Reconstitution）

Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.

存储 & 运输（Storage & Shipping）

1. Unopened kit should be stored at 2℃-8℃ upon receiving.

2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.

3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

Shipping Statement

原理（Assay Principles）

This assay kit is used to measure the levels of Anti-SARS-CoV-2 antibody IgG1 by employing an indirect ELISA format. The microplate in the kit has been pre-coated with SARS-CoV-2 Spike RBD. First add the diluted samples to the plate and wash the wells after incubation. Afterwards add HRP-Mouse anti-Human IgG1 to the plate and wash the wells after incubation. Lastly the substrate is loaded into the wells and color develops in proportion to the amount of antibody. The reaction is stopped by the addition of a stop solution and the intensity of the color can be measured at 450 nm and 630 nm. The OD Value reflects the amount of antibody bound.

Your experiment will include 4 simple steps:

a) Add your sample to the plate, take the Anti-SARS-CoV-2 antibody as Control sample. The samples and Control sample are diluted by Dilution Buffer.

b) Add diluted Secondary antibody HRP-Mouse-Anti-Human IgG3 to the plate. The Secondary antibody is diluted by Dilution Buffer.

c) Wash the plate and add TMB or other colorimetric HRP substrate.

d) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.