登录 | 注册    关注公众号  
微信公众号
搜索
 >  Antibody>Spike RBD >SPD-M305

Anti-SARS-CoV-2 Spike RBD Antibody, Mouse IgG1 (AS113) (Omicron Specific)

抗体来源(Source)

The mouse monoclonal antibody is produced from a hybridoma resulting from fusion of SP2/0 myeloma and B-lymphocytes obtained from a mouse immunized with Spike RBD. The antibody is specific against the Omicron (B.1.1.529/BA.1) variant of SARS-CoV-2, and has no binding with the spike RBD of the wild type virus and other viral lineages.

亚型(Isotype)

Mouse IgG/kappa

特异性(Specificity)

This product is a specific antibody against Spike RBD of Omicron (B.1.1.529/BA.1) variant of SARS-CoV-2. Cross-reactivity with Spike protein RBD domain of other coronaviruses, including SARS-CoV, MERS-CoV, HCoV-229E, HCoV-NL63, HCoV-OC43 and HCoV-HKU1, has not been tested.

纯度(Purity)

>95% as determined by SDS-PAGE.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Spike RBD SDS-PAGE

Anti-SARS-CoV-2 Spike RBD Antibody, Mouse IgG1 (AS113) (Omicron Specific) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Spike RBD ELISA

Immobilized SARS-CoV-2 Spike RBD, His Tag (B.1.1.529/Omicron) (Cat. No. SPD-C522e) can bind Anti-SARS-CoV-2 Spike RBD Antibody, Mouse IgG1 (AS113) (Omicron Specific) (Cat. No. SPD-M305) with a linear range of 0.4-12.5 ng/mL (QC tested). The antibody does not bind Spike RBD of WT (Cat. No. SPD-C52H1), Alpha (Cat. No. SPD-C52Hn), Beta (Cat. No. SPD-C52Hp), Delta (Cat. No. SPD-C52Hh) and Gamma (Cat. No. SPD-C52Hr).

Protocol

 

活性(Bioactivity)-BLI

Spike RBD BLI

Loaded Anti-SARS-CoV-2 Spike RBD Antibody, Mouse IgG1 (AS113) (Omicron Specific) (Cat. No. SPD-M305) on AMC Biosensor, can bind SARS-CoV-2 Spike RBD, His Tag (B.1.1.529/Omicron) (Cat. No. SPD-C522e) with an affinity constant of 9.07 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

背景(Background)

Its been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

 

 

前沿进展

 
 
货号/价格
文档
联系电话:
+86 400-682-2521(全国)
010-53681107(北京)
021-50850665(上海)
运输方式
订单邮箱:
order.cn@acrobiosystems.com
技术支持邮箱:
tech.cn@acrobiosystems.com
前沿进展
点击查看详细

消息提示

请输入您的联系方式,再点击提交!

确定