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 >  Antibody>FMC63 >FM3-FY45P1

FITC-Labeled Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (Y45) (HEK293)

相关产品(Related Products): FITC PE Biotin APC PE APC AF647

抗体来源(Source)

Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (Y45) is expressed from human HEK293 cells, which provides higher batch consistency and long term security of supply.

We carry another premium grade FITC-Labeled Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (Y45) (FM3-FY45G0), produced with the same production process but under more rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. It is designed for cell isolation and cell culture applications in the early preclinical stage.

应用(Application)

Flow Cytometry (Evaluation of Anti-CD19 (FMC63 scFv) CAR Expression).

克隆号(Clone)

Y45

种属(Species)

Mouse

亚型(Isotype)

Mouse IgG1/kappa

特异性(Specificity)

Specifically recognizes the antigen-recognition domain of FMC63 derived CARs.

免疫原(Immunogen)

Recombinant FMC63 scFv derived from HEK293 cells.

偶联(Conjugate)

FITC

Excitation source: 488 nm spectral line, argon-ion laser

Excitation Wavelength: 488 nm

Emission Wavelength: 535 nm

同型对照(Isotype Control)

The Isotype control is sold separately and you can search for Cat. No. DNP-FM1A1 for product information.

推荐稀释比(Recommended Dilution)

1:50

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, 0.5% BSA, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 24 months in lyophilized state;
  2. -70°C for 12 months under sterile conditions after reconstitution.
 

活性(Bioactivity)-FACS

FMC63 FACS

2e5 of anti-CD19 CAR-293 cells were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) of FITC-Labeled Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (Cat. No. FM3-FY45P1) and Isotype control antibody respectively. FITC signal was used to evaluate the binding activity (QC tested).

Protocol

 

背景(Background)

FMC63 is an IgG2a mouse monoclonal antibody specific for CD19, which is a target for the immunotherapy of B lineage leukaemias and lymphomas. FMC63 scFv is the most commonly used ectodomain component of CD19-specific CARs. So far, most of reported CART19 trials contain the anti-CD19 scFv derived from FMC63, including the two FDA-approved CARs Kymriah and Yescarta.

 

前沿进展

 
 
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