膜杰作
Star Staining
- Genetically modified cell lines best reflect MOA (Mechanism of Action)
- Higher activity and larger assay window for robust and reproducible cell-based bioassay
- Comprehensive application data to support assay development and validation
- Full tracible record, stringent quality control and validated cell passage stability
- Parental cell line legally obtained from internationally recognized cell resource bank and commercially licensed
- Global commercial license assistance whenever regulatory filing is required
描述（Description）
The NF-kB (Luc) HEK293 Reporter Cell was engineered with the NF-kB response element driving luciferase expressing systems. The receptors expressing endogenously or transfected on this reporter cell were activated by corresponding ligands binding, transducing intracellular signals resulting in NF-kB-RE mediated luminescence.
应用说明（Application）
1. The discovery of activators or inhibitors by the NF-kB signaling bioactivity
2. Transfection host for some receptors concerning the NF-kB signaling pathway
生长特性（Growth Properties）
Adherent
筛选标记（Selection Marker）
Puromycin (2 μg/mL)
培养基（Culture Medium）
DMEM medium + 10% FBS
冻存液（Freeze Medium）
Serum-free cell cryopreservation medium
装量（Quantity）
1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium
存储（Storage）
Frozen in liquid nitrogen.
支原体检测（Mycoplasma Testing）
Negative
无菌检测（Sterility Testing）
Negative
使用说明（Instructions for Use）
See data sheet for detailed culturing and assay protocol.
Signaling Bioassay
Response to human TNFα protein (RLU).
The NF-kB (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of human TNFα protein (AcroBiosystems, Cat. No. TNA-H4211). The EC50 was approximately 0.64 ng/mL.
Protocol
Response to human TNFα protein (Fold).
The NF-kB (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of human TNFα protein (AcroBiosystems, Cat. No. TNA-H4211). The max induction fold was approximately 235.
Protocol
Passage Stability
Passage stability analysis by Signaling Bioassay.
The continuously growing NF-kB (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of human TNFα protein. Human TNFα protein stimulated response demonstrates passage stabilization (fold induction and EC50) across passage 8-26.
Protocol
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背景（Background）
The nuclear factor-κB (NF-κB) transcription factor family, which has been considered the central mediator of the inflammatory process and a key participant in innate and adaptive immune responses, could accelerate cell proliferation, inhibit apoptosis, promote cell migration and invasion, and stimulate angiogenesis and metastasis. Notably, in many types of cancer, both in malignant cells and in the tumour microenvironment, NFκB is constitutively active. Most of the increased NF-κB activity observed in solid malignancies is due to elevated production of IKK activating cytokines, including tumour necrosis factor (TNF) and IL-1.
Limited Use&License Disclosure
- This cell line is provided for research use only. It is neither intended for any animal or human therapeutic use nor for any direct human in vivo use. You are restricted to share, modify, transfer, distribute, sell, sublicense, or otherwise make the cell line available for use to other researchers, laboratories, research institutions, hospitals, universities, or service organizations.
- ACROBIOSYSTEMS MAKES NO WARRANTIES OR REPRESENTATIONS OF ANY KIND, EITHER EXPRESSED OR IMPLIED, WITH RESPECT TO THE SUITABILITY OF THE CELL LINE FOR ANY PARTICULAR USE.
- ACROBIOSYSTEMS ACCEPTS NO LIABILITY IN CONNECTION WITH THE HANDLING OR USE OF THE CELL LINE.
- Modifications of the cell line, transfer to a third party, or commercial use of the cell line may require a separate license and additional fees. Please contact order.cn@acrobiosystems.com for further details.
