膜杰作
Star Staining
分子别名（Synonym）
IL-17F,Interleukin-17F,Cytokine ML-1
表达区间及表达系统（Source）
Biotinylated Human IL-17F, His,Avitag (ILF-H82Q9) is expressed from human 293 cells (HEK293). It contains AA Arg 31 - Gln 163 (Accession # Q96PD4-1).
Predicted N-terminus: His
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 18.5 kDa. The protein migrates as 25-28 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
标记（Labeling）
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白标记度（Protein Ratio）
Passed as determined by the HABA assay / binding ELISA.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Biotinylated Human IL-17F, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Biotinylated Human IL-17F, His,Avitag (Cat. No. ILF-H82Q9) is more than 90% and the molecular weight of this protein is around 37-55 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Human IL17RA & IL17RC Protein, Fc Tag&Fc Tag (Cat. No. ILC-H5257) at 2 μg/mL (100 μL/well) can bind Biotinylated Human IL-17F, His,Avitag (Cat. No. ILF-H82Q9) with a linear range of 0.6-78 ng/mL (QC tested).
Protocol
Immobilized Biotinylated Human IL-17F, His,Avitag (Cat. No. ILF-H82Q9) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116)precoated (0.5 μg/well) plate can bind Human IL-17 RA, Fc Tag (MALS verified) with a linear range of 1-78 ng/mL (Routinely tested).
Protocol
Immobilized Biotinylated Human IL-17F, His,Avitag (Cat. No. ILF-H82Q9) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human IL-17 RC, Fc Tag (Cat. No. ILC-H5259) with a linear range of 2-156 ng/mL (Routinely tested).
Protocol
背景（Background）
Interleukin-17F (IL17F) is also known as Interleukin-24 (IL24), Cytokine ML-1, is a secreted disulfide-linked homodimer which belongs to the IL-17 family. There are at least six members of the IL-17 family in humans and in mice: IL-17A, IL-17B, IL-17C, IL-17D, IL-17E (also called IL-25) and IL-17F (also called IL-24). IL-17 represents a family of structurally related cytokines that share a highly conserved C-terminal region but differ from one another in their N-terminal regions and in their distinct biological roles. IL-17F / IL-24 is expressed in activated, but not resting, CD4+ T-cells and activated monocytes. IL17F / Interleukin-24 stimulates the production of other cytokines such as IL-6, IL-8 and granulocyte colony-stimulating factor, and can regulate cartilage matrix turnover. IL17F / IL-24 stimulates PBMC and T-cell proliferation and Inhibits angiogenesis. Defects in IL17F are the cause of familial candidiasis type 6 (CANDF6).
