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 >  Protein>ADAM9 >AD9-H52H7

Human ADAM9 Protein, His Tag (active enzyme)

分子别名(Synonym)

Disintegrin and metalloproteinase domain-containing protein 9 (EC:3.4.24.-) Cellular disintegrin-related protein,Meltrin-gamma,Metalloprotease,disintegrin,cysteine-rich protein 9,Myeloma cell metalloproteinase,ADAM9,KIAA0021, MCMP, MDC9, MLTNG

表达区间及表达系统(Source)

Human ADAM9, His Tag (AD9-H52H7) is expressed from human 293 cells (HEK293). It contains AA Ala 206 - Asp 697 (Accession # Q13443-1).

Predicted N-terminus: Ala 206

Request for sequence

蛋白结构(Molecular Characterization)

ADAM9 Structure

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 55.2 kDa. The protein migrates as 65-70 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Supplied as 0.2 μm filtered solution in 20 mM Tris, 500 mM NaCl, pH7.5 with glycerol as protectant.

Contact us for customized product form or formulation.

运输(Shipping)

This product is supplied and shipped with dry ice, please inquire the shipping cost.

存储(Storage)

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. The product MUST be stored at -70°C or lower upon receipt;
  2. -70°C for 3 months under sterile conditions.
 

电泳(SDS-PAGE)

ADAM9 SDS-PAGE

Human ADAM9, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

 

活性(Bioactivity)-ELISA

ADAM9 ELISA

Immobilized Human ADAM9, His Tag (Cat. No. AD9-H52H7) at 1 μg/mL (100 μL/well) can bind Izeltabart with a linear range of 0.06-1 ng/mL (QC tested).

Protocol

ADAM9 ELISA

Immobilized Izeltabart at 1 μg/mL (100 μL/well) can bind Human ADAM9, His Tag (Cat. No. AD9-H52H7) with a linear range of 2-31 ng/mL (Routinely tested).

Protocol

 

活性(Bioactivity)

Measured by its ability to cleave a fluorogenic peptide substrate Mca-PLAQAV-Dpa-RSSSR-NH2. The specific activity is >10 pmol/min/µg (QC tested).

Protocol

 

背景(Background)

ADAM9 (A disintegrin and a metalloprotease 9) is a membrane-anchored protein that participates in a variety of physiological functions, primarily through the disintegrin domain for adhesion and the metalloprotease domain for ectodomain shedding of a wide variety of cell surface proteins. ADAM9 influences the developmental process, inflammation, and degenerative diseases.Recently, increasing evidence has shown that ADAM9 plays an important role in tumor biology. Overexpression of ADAM9 has been found in several cancer types and is correlated with tumoraggressiveness and poor prognosis. In addition, through either proteolytic or non-proteolytic pathways, ADAM9 promotes tumor progression, therapeutic resistance, and metastasis of cancers.Therefore, comprehensively understanding the mechanism of ADAM9 is crucial for the development of therapeutic anti-cancer strategies. In this review, we summarize the current understanding of ADAM9 in biological function, pathophysiological diseases, and various cancers. Recent advances in therapeutic strategies using ADAM9-related pathways are presented as well.

 

前沿进展

 
 
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ADAM9靶点信息
英文全称:Disintegrin and metalloproteinase domain-containing protein 9
中文全称:
种类:
上市药物数量:0详情
临床药物数量:1详情
最高研发阶段:临床二期
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