分子别名(Synonym)
TNFRSF17,CD269,BCM,BCMA
表达区间及表达系统(Source)
Cynomolgus / Rhesus macaque BCMA, Llama IgG2b Fc Tag (BCA-C5257) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ala 53 (Accession # A0A2K5UD97-1). In the region Met 1 - Ala 53, the AA sequence of Cynomolgus and Rhesus macaque BCMA are homologus.
Predicted N-terminus: Met 1
Request for sequence
蛋白结构(Molecular Characterization)
This protein carries a llama IgG2b Fc tag at the C-terminus
The protein has a calculated MW of 33.8 kDa. The protein migrates as 40-48 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素(Endotoxin)
Less than 0.01 EU per μg by the LAL method.
纯度(Purity)
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
制剂(Formulation)
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法(Reconstitution)
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储(Storage)
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳(SDS-PAGE)
Cynomolgus / Rhesus macaque BCMA, Llama IgG2b Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Cynomolgus / Rhesus macaque BCMA, Llama IgG2b Fc Tag (Cat. No. BCA-C5257) is more than 95% and the molecular weight of this protein is around 65-88 kDa verified by SEC-MALS.
Report
活性(Bioactivity)-ELISA
Immobilized Cynomolgus / Rhesus macaque BCMA, Llama IgG2b Fc Tag (Cat. No. BCA-C5257) at 5 μg/mL (100 μL/well) can bind Biotinylated Human BAFF, Avitag,Fc Tag (active trimer) (MALS verified) (Cat. No. BAF-H82F3) with a linear range of 0.1-8 ng/mL (QC tested).
Protocol
Immobilized Human APRIL, His,Flag Tag, active trimer (Cat. No. APL-H52D1) at 5 μg/mL (100 μL/well) can bind Cynomolgus / Rhesus macaque BCMA, Llama IgG2b Fc Tag (Cat. No. BCA-C5257) with a linear range of 0.1-8 ng/mL (Routinely tested).
Protocol
背景(Background)
Tumor necrosis factor receptor superfamily member 17 (TNFRSF17) is also known as B-cell maturation protein (BCMA), CD antigen CD269, which is a member of the TNF-receptor superfamily. TNFRSF17 contains one TNFR-Cys repeat. TNFRSF17 is expressed in mature B-cells, but not in T-cells or monocytes. TNFRSF17 is receptor for TNFSF13B/BLyS/BAFF and TNFSF13/APRIL. TNFRSF17 promotes B-cell survival and plays a role in the regulation of humoral immunity. TNFRSF17 can activate NF-kappa-B and JNK.