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 >  Protein>IL-4 >IL4-M52H5

Mouse IL-4 Protein, His Tag

分子别名(Synonym)

IL4,BCGF1,BSF1

表达区间及表达系统(Source)

Mouse IL-4, His Tag (IL4-M52H5) is expressed from human 293 cells (HEK293). It contains AA His 21 - Ser 140 (Accession # P07750-1).

Predicted N-terminus: His 21

Request for sequence

蛋白结构(Molecular Characterization)

IL-4 Structure

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 15.4 kDa. The protein migrates as 17-20 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

IL-4 SDS-PAGE

Mouse IL-4, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

 

活性(Bioactivity)-ELISA

IL-4 ELISA

Immobilized Mouse IL-4, His Tag (Cat. No. IL4-M52H5) at 5 μg/mL (100 μL/well) can bind Mouse IL-4 R alpha, Fc Tag (Cat. No. ILR-M5252) with a linear range of 0.2-16 ng/mL (QC tested).

Protocol

 

背景(Background)

Interleukin-4, is a cytokine that induces differentiation of naive helper T cells (Th0 cells to Th2 cells). In the presence of IL-4 and IL-13, cytokines that are produced in a Th-2 type response, particularly during allergy and parasitic infections, macrophages become differentially activated, And this cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. Recently, researcher found that the cytokine IL-4 plays a key role in development of innate CD8+ T cells in the thymus of several gene-deficient mouse strains, including Itk, KLF2, CBP and Id3, without previous exposure to antigen.

 

前沿进展

 
 
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IL-4靶点信息
英文全称:Interleukin-4
中文全称:白细胞介素-4
种类:Homo sapiens
上市药物数量:1详情
临床药物数量:8详情
最高研发阶段:批准上市
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