膜杰作
Star Staining
分子别名（Synonym）
GPRC5D,G-protein coupled receptor family C group 5 member D
表达区间及表达系统（Source）
Fluorescent Human GPRC5D Full Length Protein-VLP (GPD-HF2P7) is expressed from human 293 cells (HEK293). It contains AA Tyr 2 - Cys 300 (Accession # Q9NZD1-2).
Predicted N-terminus: Met
蛋白结构（Molecular Characterization）
This protein carries a GFP tag.
Virus-like particles(VLPs) are formed by self-assembly of envelop/capsid proteins from viruses. Membrane Proteins can be constituted in-situ with VLPs produced from HEK293 cell cultures. These VLPs concentrate conformationally intact membrane proteins directly on the cell surface and produce soluble, high-concentration proteins perfect for immunization and antibody screening.
The VLPs provide the display of properly folded membrane proteins in their native cellular membrane in a compact size of 100~300 nm diameter (similar to the size of most viruses) making it optimal targets for dendritic cells in vivo and surface attachment for phage display.
偶联（Conjugate）
GFP
Excitation source: 488 nm spectral line, argon-ion laser
Excitation Wavelength: 488 nm
Emission Wavelength: 530 nm
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
制剂（Formulation）
The VLPs are highly immunogenic, so the immunization strategy should be optimized (antigen dose, regimen and adjuvant).
Supplied as 0.2 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
运输（Shipping）
This product is supplied and shipped with dry ice, please inquire the shipping cost.
存储（Storage）
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 12 months under sterile conditions.
活性（Bioactivity）-ELISA
Immobilized Fluorescent Human GPRC5D Full Length Protein-VLP (Cat. No. GPD-HF2P7) at 1 μg/mL (100 μL/well) can bind Anti-GPRC5D Antibody, Human IgG4 with a linear range of 0.002-0.313 μg/mL (QC tested).
Protocol
活性（Bioactivity）-FACS
2e5 of Anti-GPRC5D CAR-293 Cells were stained with 100 μL of 10 μg/mL of Fluorescent Human GPRC5D Full Length Protein (VLP) (Cat. No. GPD-HF2P7) and isotype control protein respectively. GFP signal was used to evaluate the binding activity (QC tested).
Protocol
均一性（Identity）-DLS
The mean peak Radius of VLP is 65-85 nm with more than 95% intensity as determined by dynamic light scattering (DLS).
背景（Background）
G-protein coupled receptor family C group 5 member D (GPRC5D) is a retinoic acid-inducible 40 kDa protein with seven transmembrane segments and a short N terminal extracellular region. Widely expressed in the peripheral system. Expression pattern is high in pancreas, medium in kidney, small intestine, spleen and testis, low in lung, colon, leukocyte, prostate and thymus and not detectable in brain, heart, liver, placenta, skeletal muscle and ovary.
