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 >  Protein>Flt-3 >FL3-HF255

FITC-Labeled Human Flt-3 / Flk-2 Protein, Fc Tag

分子别名(Synonym)

Flt-3,Flk-2,STK-1,CD135,FLK2,FLT-3

表达区间及表达系统(Source)

FITC-Labeled Human Flt-3, Fc Tag (FL3-HF255) is expressed from human 293 cells (HEK293). It contains AA Asn 27 - Ser 543 (Accession # P36888-1).

Predicted N-terminus: Asn 27

Request for sequence

蛋白结构(Molecular Characterization)

Flt-3 Structure

This protein carries a human IgG1 Fc tag at the C-terminus

The protein has a calculated MW of 84.8 kDa. The protein migrates as 110-130 kDa and 250 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

偶联(Conjugate)

FITC

Excitation source: 488 nm spectral line, argon-ion laser

Excitation Wavelength: 488 nm

Emission Wavelength: 535 nm

标记(Labeling)

The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular sieve treatment during purification process.

蛋白标记度(Protein Ratio)

The FITC to protein molar ratio is 2-4.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Flt-3 SDS-PAGE

FITC-Labeled Human Flt-3, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

 

活性(Bioactivity)-ELISA

Flt-3 ELISA

Immobilized Human Flt-3 Ligand, premium grade (Cat. No. FLL-H5218) at 1 μg/mL (100 μL/well) can bind FITC-Labeled Human Flt-3, Fc Tag (Cat. No. FL3-HF255) with a linear range of 0.2-13 ng/mL (QC tested).

Protocol

 

背景(Background)

Flt-3(Receptor-type tyrosine-protein kinase FLT3) is also known as FLK-2(Fetal liver kinase-2), STK-1(Stem cell tyrosine kinase 1), CD135. FLT3 is a cytokine receptor which belongs to the receptor tyrosine kinase class III. Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation and survival of hematopoietic progenitor cells and of dendritic cells. Promotes phosphorylation of SHC1 and AKT1, and activation of the downstream effector MTOR. Promotes activation of RAS signaling and phosphorylation of downstream kinases, including MAPK1/ERK2 and/or MAPK3/ERK1. Mutations that cause constitutive kinase activity promote cell proliferation and resistance to apoptosis via the activation of multiple signaling pathways.

 

前沿进展

 
 
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Flt-3靶点信息
英文全称:Tyrosine-protein kinase receptor FLT3
中文全称:酪氨酸蛋白激酶受体FLT3
种类:Homo sapiens
上市药物数量:13详情
临床药物数量:46详情
最高研发阶段:批准上市
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