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Biotinylated Human DDR1 Protein, His,Avitag™ (MALS verified)

分子别名(Synonym)

CD167a

表达区间及表达系统(Source)

Biotinylated Human DDR1, His,Avitag (DD1-H82E9) is expressed from human 293 cells (HEK293). It contains AA Asp 21 - Thr 416 (Accession # Q08345-1).

Predicted N-terminus: Asp 21

Request for sequence

蛋白结构(Molecular Characterization)

Online(Asp 21 - Thr 416) Q08345-1

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 47.5 kDa. The protein migrates as 50-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Biotinylation)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Biotin:Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Biotinylated Human DDR1, His,Avitag (Cat. No. DD1-H82E9) SDS-PAGE gel

Biotinylated Human DDR1, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

Biotinylated Human DDR1, His,Avitag (Cat. No. DD1-H82E9) MALS images

The purity of Biotinylated Human DDR1, His,Avitag (Cat. No. DD1-H82E9) is more than 90% and the molecular weight of this protein is around 51-69 kDa verified by SEC-MALS.

Report

活性(Bioactivity)-BLI

Biotinylated Human BLI

Loaded Biotinylated Human DDR1, His,Avitag (Cat. No. DD1-H82E9) on SA Biosensor, can bind Native Human Collagen I protein with an affinity constant of 0.603 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).

Protocol

 

背景(Background)

Discoidin domain receptor 1 (DDR1) is a member of DDRs, which are members of the receptor tyrosine kinase (RTK). Upon collagen binding, DDR1 undergoes tyrosine autophosphorylation, which consequently triggers downstream genetic and cellular pathways and plays critical roles in the regulation of cellular morphogenesis, differentiation, proliferation, adhesion, migration, and invasion. Research shows that DDR1 is closely related to various human diseases including cancer, fibrosis, atherosclerosis, and other inflammatory disorders. New generation DDR1 inhibitors targeting the allosteric sites outside of the canonical ATP-binding pocket or extracellular domain (allosteric inhibitors) may offer a new opportunity for selective DDR1 inhibition therapy development.

 

 

前沿进展

 
 
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DDR1靶点信息
英文全称:Discoidin domain receptor 1
中文全称:盘状结构域受体-1
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:6详情
最高研发阶段:临床二期
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