膜杰作
Star Staining
| ID | Components | Size |
| RAS081-C01 | Pre-coated Anti-SARS-CoV-2 Spike Trimer (B.1.1.529) Antibody Microplate | 1 plate |
| RAS081-C02 | SARS-CoV-2 Spike Trimer (B.1.1.529) | 10 μg |
| RAS081-C03 | HRP-Anti-SARS-CoV-2 Spike Trimer Antibody | 20 μg |
| RAS081-C04 | 10xWashing Buffer | 50 mL |
| RAS081-C05 | Dilution Buffer | 50 mL |
| RAS081-C06 | Substrate Solution | 12 mL |
| RAS081-C07 | Stop Solution | 7 mL |
背景（Background）
The newly identified Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has posed a serious threat to human health. A rapid and effective assay kit detecting the levels of SARS-CoV-2 Spike protein is urgently needed to accelerate the development of COVID-19 vaccines.
应用说明（Application）
The kit is developed for specific detection of SARS-CoV-2 Spike Protein (B.1.1.529) in vaccine samples, which can meet the needs of vaccine developers to establish antigen quantification methods for preclinical evaluation, vaccine production and quality control,and realize accurate quantification of vaccine antigen contents for COVID-19 vaccines of Omicron-specific boosters.
It is for research use only.
重构方法（Reconstitution）
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
存储 & 运输（Storage & Shipping）
1. Unopened kit should be stored at 2℃-8℃ upon receiving.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
原理（Assay Principles）
This assay kit employs a standard sandwich-ELISA format, providing a rapid detection of SARS-CoV-2 Spike Protein. The kit consists of microplate pre-coated with Anti-SARS-CoV-2 Spike protein Antibody, an SARS-CoV-2 Spike Protein as Control, an HPR-Anti-SARS-CoV-2 Spike protein Antibody, and buffers.
Your experiment will include 5 simple steps:
a) All reagents were returned to room temperature(20°C-25°C) before use.
b) Add your sample to the plate, take the SARS-CoV-2 Spike protein as Control sample. The samples and Control sample are diluted by Dilution Buffer.
c) Add a diluted HRP-Anti-SARS-CoV-2 Spike Protein Antibody to the plate. The diluted by Dilution Buffer.
d) Wash the plate and add TMB.
e) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of protein bound.
典型数据-Typical Data Please refer to Ds document for the assay protocol.
Detection of SARS-CoV-2 Spike Trimer (B.1.1.529) by sandwich-ELISA Assay.
Immobilized Anti-SARS-CoV-2 Spike Trimer (B.1.1.529) Antibody at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 Spike Trimer (B.1.1.529). Detection was performed using HRP-Anti-SARS-CoV-2 Spike Trimer Antibody with sensitivity of 23 ng/mL (QC tested).
SARS-CoV-2 Spike Trimer (B.1.1.529) Specific ELISA Kit (For Vaccine Development)(Cat.No.RAS-A081) can specifically detect Omicron(SPN-C52Hz),cannot detect other strains,inchuding WT, Alpha, Beta, Gamma and Delta.
