膜杰作
Star Staining
| ID | Components | Size |
| RAS091-C01 | Pre-coated SARS-CoV-2 Spike RBD (B.1.617.2) Microplate | 1 plate |
| RAS091-C02 | SARS-CoV-2 Antibody Positive Control | 100 μL |
| RAS091-C03 | SARS-CoV-2 Antibody Negative Control | 100 μL |
| RAS091-C04 | HRP-Conjugated Antibody | 10 μg |
| RAS091-C05 | 10xWashing Buffer | 50 mL |
| RAS091-C06 | Dilution Buffer | 50 mL |
| RAS091-C07 | Substrate Solution | 12 mL |
| RAS091-C08 | Stop Solution | 7 mL |
背景（Background）
The B.1.617.2 (Delta) variant of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was first identified in the state of Maharashtra in late 2020 and spread throughout India, outcompeting pre-existing lineages including B.1.617.1 (Kappa) and B.1.1.7 (Alpha)1. In vitro, B.1.617.2 is sixfold less sensitive to serum neutralizing antibodies from recovered individuals, and eightfold less sensitive to vaccine-elicited antibodies, compared with wild-type Wuhan-1 bearing D614G. To facilitate the mutant-related research, drug trials and vaccine development, a high-throughput assay to measure IgG antibodies against the mutants is in urgent need.
应用说明（Application）
This kit is developed for titer measurement of Anti-SARS-CoV-2 (B.1.617.2) Antibody IgG in Mouse serum.
It is for research use only.
重构方法（Reconstitution）
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
存储 & 运输（Storage & Shipping）
1. Unopened kit should be stored at 2℃-8℃ upon receiving.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
原理（Assay Principles）
This assay kit employs a standard indirect-ELISA format, providing a rapid detection of Anti-SARS-CoV-2 antibodies in Mouse serum by SARS-CoV-2 Spike RBD . The Kit consists of Pre-coated with SARS-CoV-2 Spike RBD Microplate, an Positive Control, an Negative Control, an HRP-Anti-Goat anti mouse IgG secondary antibody.
Your experiment will include 4 simple steps:
a) The samples and Control are diluted by Dilution Buffer.Add your sample to the plate.
b) Add diluted Secondary antibody HRP-Goat anti-Mouse IgG to the plate. The Secondary antibody is diluted by Dilution Buffer.
c) Wash the plate and add TMB or other colorimetric HRP substrate.
d) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.
