膜杰作
Star Staining
& Cat.No. | Product | Size | Amount |
| Human T cell Activation/Expansion CD3/CD28 Beads, premium grade DMF Filed | 2.5 mg | 2.5 × 10⁷ beads |
| 10 mg | 1.0 × 10⁸ beads |
背景（Background）
Human T cell Activation/Expansion CD3/CD28 Beads, premium grade DMF Filed are uniform 5.5 μm of magnetic beads coated with an optimized mixture of monoclonal antibodies against the CD3 and CD28. The CD3 antibody is specific for the epsilon chain of human CD3, which is considered to be a subunit of the TCR complex. The CD28 antibody is specific for the human CD28 co-stimulatory molecule, which is the receptor for CD80 (B7-1) and CD86 (B7-2). Both antibodies are mouse anti-human IgGs coupled to the same bead, mimicking in vivo stimulation by APCs.
应用说明（Application）
ActiveMax® Human T cell Activation/Expansion CD3/CD28 Beads, premium grade DMF Filed is intended for the in vitro isolation, stimulation and expansion of purified T cell populations of, for example, CD3+T cells, CD4+ T cells, CD8+ T cells or human PBMC in early preclinical stage.
重构方法（Reconstitution）
See Certificate of Analysis (CoA) for detailed instruction.
存储（Storage）
The product is shipped at ambient temperature. Upon receipt, store it immediately at -20°C or below for long term storage. This product is stable after storage at: 1. -20°C or below for 5 years in lyophilized state from the date of receipt; 2. 2-8°C for 12 months under sterile conditions after reconstitution.
Please avoid repeated freeze-thaw cycles after reconstitution.
无菌（Sterility）
Negative
内毒素（Endotoxin）
Less than 0.002 EU per μg by the LAL method.
注意事项（Important Note）
This product is for research use only and not intended for therapeutic or in vivo diagnostic use.
制剂（Formulation）
Please contact us for detailed information.
Contact us for customized product form or formulation.
典型数据-Typical Data Please refer to DS document for the assay protocol.
Fig.1. Activation of the purified human T Cells.The human T cells were stimulated with ActiveMax® Human T cell Activation/Expansion CD3/CD28 Beads, premium grade(Cat. No. MBS-C001) for 24hrs, and the activation was assessed by measuring expression of both activation markers CD25 and CD69 expression on the T cells surface by stanning with PE labeled anti-human CD25 antibody and FITC labeled anti-human CD69 antibody respectively (QC tested).
Fig.2. Purified human T Cells expansion.The human T cells were labeled with carboxy fluorescein succinimidyl ester (CFSE) and stimulated with ActiveMax® Human T cell Activation/Expansion CD3/CD28 Beads, premium grade(Cat. No. MBS-C001), and then the proliferation of the T cells was assessed with CFSE dilution assay by flow cytometry on day 5 after stimulation (QC tested).
稳定性（Stability）
Activation of the purified human T Cells. The purified human T cells were activated using Human T cell Activation/Expansion CD3/CD28 Beads, (ACRO, Cat. No. GMP-MBS001/MBS-C001) respectively for 24 hours with CTS Optimizer Medium. Cells were fluorescently stained using PE labeled anti-human CD25 antibody and labeled FITC anti-human CD69 antibody and analyzed by flow cytometry.
Expansion of the human CD3+T cells. Human T cells using ACROBiosystems CD3/CD28 Beads (ACRO, Cat. No. GMP-MBS001/MBS-C001) were expanded under two different medium, respectively. Expansion was performed for two weeks, showing that ACROBiosystems’ GMP and PG beads showing similar proliferative abilities.
PD-1 expression of the activated human T Cells. The purified human T cells were stimulated using Human T cell Activation/Expansion CD3/CD28 Beads at a ratio of 1:1 beads-to-cells. Cells were expanded in T cell culture medium supplemented with 4ng/mL of rhIL-2 Protein. Activated T cells were expanded for up to 8 days with low PD-1 expression.
