膜杰作
Star Staining
分子别名（Synonym）
IL-2 R beta & IL-2 R alpha & IL-2 R gamma
表达区间及表达系统（Source）
Biotinylated Human IL-2RB&IL-2RA&IL-2RG, His,Avitag&Twin Strep Tag (ILG-H82W9) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Asp 239 (IL-2RB) & Glu 22 - Ser 212 (IL-2RA) & Leu 23 - Asn 254 (IL-2RG) (Accession # P14784-1 (IL-2RB) & P01589-1 (IL-2RA) & P31785-1 (IL-2RG)).
Predicted N-terminus: Ala 27 (IL-2RB) & Glu 22 (IL-2RA)
Request for sequence
蛋白结构（Molecular Characterization）
Biotinylated Human IL-2RB&IL-2RA&IL-2RG, His,Avitag&Twin Strep Tag is produced by co-expression of IL-2RB and IL-2RA and IL-2RG, has a calculated MW of 31.5 kDa (IL-2RB) & 55.8 kDa (IL-2RA&IL-2RG). Subunit IL-2RB is fused with a his tag at the C-terminus, followed by a Avi tag (Avitag™) and subunit IL-2RA&IL-2RG is fused with a Twin-Strep Tag at the C-terminus. The reducing (R) protein migrates as 44 kDa,46 kDa and 80-90 kDa respectively due to glycosylation.
标记（Labeling）
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白标记度（Protein Ratio）
Passed as determined by the HABA assay / binding ELISA.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Biotinylated Human IL-2RB&IL-2RA&IL-2RG, His,Avitag&Twin Strep Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS
The purity of Biotinylated Human IL-2RB&IL-2RA&IL-2RG, His,Avitag&Twin Strep Tag (Cat. No. ILG-H82W9) is more than 85% and the molecular weight of this protein is around 155-190 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Human IL-2 Protein, Fc Tag (Cat. No. IL2-H5269) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-2RB&IL-2RA&IL-2RG, His,Avitag&Twin Strep Tag (Cat. No. ILG-H82W9) with a linear range of 0.3-39 ng/mL (QC tested).
Protocol
活性（Bioactivity）-SPR
Biotinylated Human IL-2RB&IL-2RA&IL-2RG, His,Avitag&Twin Strep Tag (Cat. No. ILG-H82W9) captured on Biotin CAP-Series S Sensor Chip can bind Human IL-2 Protein, Tag Free with an affinity constant of 35.1 pM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
背景（Background）
Both Interleukin-2 receptor subunit beta and Interleukin-2 receptor subunit gamma are receptor for interleukin-2. Common subunit for the receptors for a variety of interleukins. Interacts with SHB upon interleukin stimulation. Probably in association with IL15RA, involved in the stimulation of neutrophil phagocytosis by IL15. This beta subunit is involved in receptor mediated endocytosis and transduces the mitogenic signals of IL2. IL2R exists in 3 different forms: a high affinity dimer, an intermediate affinity monomer (beta subunit), and a low affinity monomer (alpha subunit). The high and intermediate affinity forms also associate with a gamma subunit.
