登录 | 注册    关注公众号  
微信公众号
搜索
 >  Protein>GSK-3beta >GSB-H5545

Human GSK-3beta / GSK-3β Protein, His Tag (active enzyme, HPLC verified)

分子别名(Synonym)

GSK3B,Glycogen synthase kinase-3 beta

表达区间及表达系统(Source)

Human GSK-3beta, His Tag (GSB-H5545) is expressed from Baculovirus-Insect cells. It contains AA Met 1 - Thr 420 (Accession # P49841-1).

Predicted N-terminus: Met 1

Request for sequence

蛋白结构(Molecular Characterization)

GSK-3beta Structure

This protein carries a polyhistidine tag at the N-terminus

The protein has a calculated MW of 48.8 kDa. The protein migrates as 49-53 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

>90% as determined by SEC-HPLC.

制剂(Formulation)

Supplied as 0.2 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with glycerol as protectant.

Contact us for customized product form or formulation.

运输(Shipping)

This product is supplied and shipped with dry ice, please inquire the shipping cost.

存储(Storage)

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. The product MUST be stored at -70°C or lower upon receipt;
  2. -70°C for 3 months under sterile conditions.
 

电泳(SDS-PAGE)

GSK-3beta SDS-PAGE

Human GSK-3beta, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-HPLC

GSK-3beta SEC-HPLC

The purity of Human GSK-3beta, His Tag (Cat. No. GSB-H5545) was greater than 90% as determined by SEC-HPLC.

 

活性(Bioactivity)

The specific activity of GSK-3beta was determined to be >120 pmol/min/ug by ADP-Glo™ Kinase Assay using a synthetic substrate (YRRAAVPPSPSLSRHSSPHQ(pS)EDEEE) (QC tested).

Protocol

 

背景(Background)

Glycogen synthase kinase-3 (GSK-3) is a pivotal molecule in the development of Alzheimer's disease (AD). GSK-3beta is involved in the formation of paired helical filament (PHF)-tau, which is an integral component of the neurofibrillary tangle (NFT) deposits that disrupt neuronal function, and a marker of neurodegeneration in AD.

 

前沿进展

 
 
货号/价格
文档
联系电话:
+86 400-682-2521(全国)
010-53681107(北京)
021-50850665(上海)
运输方式
订单邮箱:
order.cn@acrobiosystems.com
技术支持邮箱:
tech.cn@acrobiosystems.com
GSK-3beta靶点信息
英文全称:Glycogen synthase kinase-3 beta
中文全称:糖原合酶激酶-3β
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:4详情
最高研发阶段:临床三期
查看更多信息
前沿进展
点击查看详细
相关产品
View All GSK-3beta

消息提示

请输入您的联系方式,再点击提交!

确定