膜杰作
Star Staining
分子别名（Synonym）
Neuron-specific enolase,gamma-enolase
表达区间及表达系统（Source）
Mouse NSE, His Tag (NSE-M5147) is expressed from E. coli cells. It contains AA Met 1 - Met 431 (Accession # Q545V3-1).
Predicted N-terminus: Met
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the N-terminus
The protein has a calculated MW of 49.3 kDa. The protein migrates as 47-50 kDa under reducing (R) condition (SDS-PAGE).
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 0.5 M Arginine, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Mouse NSE, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Mouse NSE, His Tag (Cat. No. NSE-M5147) is more than 95% and the molecular weight of this protein is around 100-115 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Mouse NSE, His Tag (Cat. No. NSE-M5147) at 1 μg/mL (100 μL/well) can bind Anti-NSE Antibody with a linear range of 0.004-0.125 μg/mL (QC tested).
Protocol
背景（Background）
Neuron-specific enolase (NSE) is known to be a cell specific isoenzyme of the glycolytic enzyme enolase. It is also known as
gamma-enolase or nolase 2 (ENO2). It has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. NSE is a highly specific marker for neurons and peripheral neuroendocrine cells. In clinical, NSE could be used as a biomarker for neuronal injury.
