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 >  Protein>NSE >NSE-M5147

Mouse NSE Protein, His Tag (MALS verified)

分子别名(Synonym)

Neuron-specific enolase,gamma-enolase

表达区间及表达系统(Source)

Mouse NSE, His Tag (NSE-M5147) is expressed from E. coli cells. It contains AA Met 1 - Met 431 (Accession # Q545V3-1).

Predicted N-terminus: Met

Request for sequence

蛋白结构(Molecular Characterization)

NSE Structure

This protein carries a polyhistidine tag at the N-terminus

The protein has a calculated MW of 49.3 kDa. The protein migrates as 47-50 kDa under reducing (R) condition (SDS-PAGE).

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 0.5 M Arginine, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

NSE SDS-PAGE

Mouse NSE, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

NSE SEC-MALS

The purity of Mouse NSE, His Tag (Cat. No. NSE-M5147) is more than 95% and the molecular weight of this protein is around 100-115 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

NSE ELISA

Immobilized Mouse NSE, His Tag (Cat. No. NSE-M5147) at 1 μg/mL (100 μL/well) can bind Anti-NSE Antibody with a linear range of 0.004-0.125 μg/mL (QC tested).

Protocol

 

背景(Background)

Neuron-specific enolase (NSE) is known to be a cell specific isoenzyme of the glycolytic enzyme enolase. It is also known as
gamma-enolase or nolase 2 (ENO2). It has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. NSE is a highly specific marker for neurons and peripheral neuroendocrine cells. In clinical, NSE could be used as a biomarker for neuronal injury.

 

前沿进展

 
 
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NSE靶点信息
英文全称:Gamma-enolase
中文全称:γ-烯醇化酶
种类:
上市药物数量:0详情
临床药物数量:0详情
最高研发阶段:临床前
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