膜杰作
Star Staining
分子别名（Synonym）
Neurofilament light polypeptide
表达区间及表达系统（Source）
Human NFL, His Tag (NFL-H5143) is expressed from E. coli cells. It contains AA Ser 2 - Asp 543 (Accession # P07196-1).
Predicted N-terminus: Met
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 63.4 kDa.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Supplied as 0.2 μm filtered solution in PBS, pH7.4 with glycerol as protectant.
Contact us for customized product form or formulation.
运输（Shipping）
This product is supplied and shipped with dry ice, please inquire the shipping cost.
存储（Storage）
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 12 months under sterile conditions.
电泳（SDS-PAGE）
The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
活性（Bioactivity）-ELISA
Immobilized Human NFL, His Tag (Cat. No. NFL-H5143) at 1 μg/mL (100 μL/well) can bind Anti NEFL Antibody with a linear range of 1-16 ng/mL (QC tested).
Protocol
背景（Background）
Neurofilaments (NF) are classed as type IV intermediate filaments found in the cytoplasm of neurons. Together with microtubules and microfilaments, they form the neuronal cytoskeleton. Neurofilaments usually contain three intermediate filament proteins: NFL, NFM, and NFH which are involved in the maintenance of neuronal caliber. Neurofilament heavy (NFH) can server as biomarkers for several diseases by indicating axonal injury.
