登录 | 注册    关注公众号  
微信公众号
搜索
 >  Protein>B7-H4 >B74-R52H9

Rat B7-H4 Protein, His Tag

分子别名(Synonym)

B7-H4,VTCN1,B7S1,B7h.5

表达区间及表达系统(Source)

Rat B7-H4, His Tag (B74-R52H9) is expressed from human 293 cells (HEK293). It contains AA Phe 29 - Gly 257 (Accession # Q501W4-1).

Predicted N-terminus: Phe 29

Request for sequence

蛋白结构(Molecular Characterization)

B7-H4 Structure

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 27.1 kDa. The protein migrates as 45-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

B7-H4 SDS-PAGE

Rat B7-H4, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

 

活性(Bioactivity)-ELISA

B7-H4 ELISA

Immobilized Rat B7-H4, His Tag (Cat. No. B74-R52H9) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-Human B7-H4 Antibody, Human IgG1 with a linear range of 0.1-8 ng/mL (QC tested).

Protocol

 

背景(Background)

V-set domain-containing T-cell activation inhibitor 1 (VTCN1) is also known as Immune costimulatory protein B7-H4, Protein B7S1, T-cell costimulatory molecule B7x, B7H4, which belongs to the immunoglobulin superfamily and BTN/MOG family. VTCN1 contains two Ig-like V-type (immunoglobulin-like) domains. The expression of VTCN1 is up-regulated by IL6 and IL10 and is inhibited by GM-CSF and IL4 on antigen-presenting cells (APCs). VTCN1 / B7-H4 negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. VTCN1 involved in promoting epithelial cell transformation.

 

前沿进展

 
 
货号/价格
文档
联系电话:
+86 400-682-2521(全国)
010-53681107(北京)
021-50850665(上海)
运输方式
订单邮箱:
order.cn@acrobiosystems.com
技术支持邮箱:
tech.cn@acrobiosystems.com
B7-H4靶点信息
英文全称:V-set domain-containing T-cell activation inhibitor 1
中文全称:含V区域T细胞激活抑制因子1
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:11详情
最高研发阶段:临床二期
查看更多信息
前沿进展
点击查看详细

消息提示

请输入您的联系方式,再点击提交!

确定