分子别名(Synonym)
Spike,S protein,Spike glycoprotein,S glycoprotein
表达区间及表达系统(Source)
Bat coronavirus Spike Trimer, His Tag&Twin-Strep Tag (SPN-B5283) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Gln 1200 (Accession # UAY13253.1 (K978P, V979P)).
Predicted N-terminus: Met 1
Request for sequence
蛋白结构(Molecular Characterization)
This protein carries a polyhistidine tag at the C-terminus, followed by a twin strep tag.
The protein has a calculated MW of 141.2 kDa. The protein migrates as 170-190 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素(Endotoxin)
Less than 1.0 EU per μg by the LAL method.
纯度(Purity)
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂(Formulation)
Lyophilized from 0.22 μm filtered solution in PBS with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法(Reconstitution)
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储(Storage)
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳(SDS-PAGE)
Bat coronavirus Spike Trimer, His Tag&Twin-Strep Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Bat coronavirus Spike Trimer, His Tag&Twin-Strep Tag (Cat. No. SPN-B5283) is more than 90% and the molecular weight of this protein is around 545-610 kDa verified by SEC-MALS.
Report
活性(Bioactivity)-ELISA
Immobilized Bat coronavirus Spike Trimer, His Tag&Twin-Strep Tag (Cat. No. SPN-B5283) at 1 μg/mL (100 μL/well) can bind Human ACE2, Fc Tag (Cat. No. AC2-H5257) with a linear range of 0.1-4 ng/mL (QC tested).
Protocol
Immobilized Bat coronavirus Spike Trimer, His Tag&Twin-Strep Tag (Cat. No. SPN-B5283) at 1 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (Cat. No. S1N-M122) with a linear range of 0.1-2 ng/mL (Routinely tested).
Protocol
背景(Background)
The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.