膜杰作
Star Staining
分子别名（Synonym）
Interleukin-17B,Cytokine Zcyto7,Interleukin-20,IL-20,Neuronal interleukin-17-related factor,IL17B,IL20, NIRF, ZCYTO7
表达区间及表达系统（Source）
Human IL-17B, Fc Tag (ILB-H5257) is expressed from human 293 cells (HEK293). It contains AA Gln 21 - Phe 180 (Accession # Q9UHF5-1).
Predicted N-terminus: Gln 21
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 44.6 kDa. The protein migrates as 50-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
电泳（SDS-PAGE）
Human IL-17B, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Human IL-17B, Fc Tag (Cat. No. ILB-H5257) is more than 85% and the molecular weight of this protein is around 90-105 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Human IL-17B, Fc Tag (Cat. No. ILB-H5257) at 1 μg/mL (100 μL/well) can bind Anti-Human IL17B Monoclonal Antibody with a linear range of 0.1-2 ng/mL (Routinely tested).
Protocol
- 176XXXXXXX7
- ACRO的IL-2系列的蛋白用了好几种tagd~每种标签都很奈斯，结合活性结果重现性很好，官方的PROTOCOL条件很精准~基本重现没有太多误差。好评
- 2023-2-22
- 153XXXXXXX3
- 我们用此蛋白用于小鼠体内动物实验，探讨重组IL2在小鼠体内实验的最大耐受剂量以及有效性，作为一个对标分子，数据持续更新中，并且结果满足期待，Acro蛋白质量很高，值得信赖，推荐。
- 2023-7-6
产品推荐（Recommended Products）
背景（Background）
IL-17B is a member of IL17 famaily, was originally described as a proinflammatory cytokine. IL-17B is produced by neutrophils and potentiates TNF-α effect on the production of cytokines and chemokines, such as IL-6, G-CSF, and CCL20, known to control immune cell trafficking to inflamed tissues. Interestingly, although IL-17B and IL-17E (IL-25) share a common receptor, IL-17RB, IL-17B, and IL-17E deficiency lead to opposite results in a model of acute colitis induced by dextran sulfate sodium. IL-17B inhibits IL-17E binding to IL-17RA– IL-17RB complexes on epithelial cells, and limits IL-17E-induced IL-6 production by colon epithelial cells.
