膜杰作
Star Staining
分子别名（Synonym）
Atrial natriuretic peptide receptor 1,NPR1,NPRA,GC-A,ANPRA,NPR-A
表达区间及表达系统（Source）
Human NPR1, Fc Tag (NP1-H5259) is expressed from human 293 cells (HEK293). It contains AA Gly 33 - Glu 473 (Accession # P16066-1).
Predicted N-terminus: Gly 33
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 75.3 kDa. The protein migrates as 95-120 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Human NPR1, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Human NPR1, Fc Tag (Cat. No. NP1-H5259) is more than 90% and the molecular weight of this protein is around 165-190 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Human NPR1, Fc Tag (Cat. No. NP1-H5259) at 5 μg/mL (100 μL/well) can bind Anti-NPR1 antibody with a linear range of 0.6-20 ng/mL (Routinely tested).
Protocol
背景（Background）
Receptor for the atrial natriuretic peptide NPPA/ANP and the brain natriuretic peptide NPPB/BNP which are potent vasoactive hormones playing a key role in cardiovascular homeostasis. Has guanylate cyclase activity upon binding of the ligand.
