Nucleocapsid protein,NP,Protein N
SARS-CoV-2 Nucleocapsid protein, His Tag (BA.5/Omicron) (NUN-C52Hx) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ala 419 (Accession # QHO62115.1(P13L, ERS31-33del, E136D, R203K, G204R, S413R)). The mutations are identified on the SARS-CoV-2 Omicron variant (Pango lineage: BA.5; GISAID clade: GRA).
Predicted N-terminus: Met 1
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 47.0 kDa.
Less than 1.0 EU per μg by the LAL method.
Lyophilized from 0.22 μm filtered solution in PBS, 0.2M Arginine, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Nucleocapsid (N) protein is the most abundant protein found in coronavirus. CoV N protein is a highly immunogenic phosphoprotein important for viral genome replication and modulation of cell signaling pathways. It was first identified by a research team while they were screening for ADP-ribosylated proteins during coronavirus (CoV) infection (Grunewald M. E., et al. 2017, Virology; 517: 62-68). The array of diverse functional activities accommodated in N protein makes it more than a structural protein but also an interesting target in the development of antiviral therapeutics. Because of the conservation of N protein sequence and its strong immunogenicity, N protein of coronavirus is chosen as a diagnostic tool.