膜杰作
Star Staining
| ID | Components | Size |
| RAS112-C01 | Pre-coated SARS-CoV-2 Spike Trimer(BA.5) Microplate | 1 plate |
| RAS112-C02 | SARS-CoV-2 Antibody Positive Control | 100 μL |
| RAS112-C03 | SARS-CoV-2 Antibody Negative Control | 100 μL |
| RAS112-C04 | HRP-Goat anti-Mouse IgG | 50 μL |
| RAS112-C05 | 10 x Washing Buffer | 50 mL |
| RAS112-C06 | Dilution Buffer | 50 mL |
| RAS112-C07 | Substrate Solution | 12 mL |
| RAS112-C08 | Stop Solution | 7 mL |
背景（Background）
Since December 2019, the emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its associated disease, COVID-19, has caused a devastating pandemic worldwide.As the virus spreads globally, the continuous emergence of new mutant strains escalated the challenge on humans.To facilitate the mutant-related research, drug trials and vaccine development, a high-throughput assay to measure IgG antibodies against the mutants is in urgent need.
应用说明（Application）
The kit is developed for titer measurement of Anti-SARS-CoV-2 (BA.5) IgG antibody (Spike Trimer) in Mouse serum.
It is for research use only.
存储 & 运输（Storage & Shipping）
1. Unopened kit should be stored at 2℃-8℃ upon receiving.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
原理（Assay Principles）
This assay kit employs a standard indirect-ELISA format, providing a rapid detection of Anti-SARS-CoV-2 antibodies in Mouse serum by SARS-CoV-2 Spike Trimer. The Kit consists of Pre-coated with SARS-CoV-2 Spike Trimer (BA.5) Microplate, an Positive Control, an Negative Control, an HRP-Anti-Goat anti mouse IgG secondary antibody.
Your experiment will include 4 simple steps:
a) The samples and Control are diluted by Dilution Buffer. Add your sample to the plate.
b) Add diluted Secondary antibody HRP-Goat anti-Mouse IgG to the plate. The Secondary antibody is diluted by Dilution Buffer.
c) Wash the plate and add TMB or other colorimetric HRP substrate.
d) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.
典型数据-Typical Data Please refer to Ds document for the assay protocol.
For determination of antibody titer: the positive sample was diluted with a gradient, and the antibody titer of the sample corresponds to the highest dilution factor that still yields a positive reading.
