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ClinMax™ Human Tumor Necrosis Factor Alpha (TNF-α) ELISA Kit

For research use only.

产品概述(Product Details)

ClinMax™ Human Tumor Necrosis Factor Alpha(TNF-α) ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant human TNF-alpha that is present in biological samples, such as human serum, plasma, and cell culture supernatants. Our ClinMax™ ELISA Kit provides several benefits:

  1. Standards to calibrate with NIBSC/WHO standards for comparable results.
  2. Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
  3. High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
  4. Simplified and straightforward protocols and ready-to-use reagents to save assay time.

应用说明(Application)

The kit is developed for quantitative detection of natural and recombinant human TNF-alpha in serum, plasma and cell culture supernatants.

It is for research use only.

流程图(Workflow)

TNF-alpha Workflow

关键信息(Key Features)

AnalyteTNF-α
Assay TypeSandwich-ELISA
ReactivityHuman
Sensitivity2.321 pg/mL
Range19.5 pg/mL-1250 pg/mL
Assay Time2 hr 50 min
Sample TypeCell Culture Supernatants, Plasma, Serum.
Sample volume100 μL
Format96-wells plate breakable into 12 x 8 wells strips

Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.

存储(Storage)

Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

 

典型数据-Typical Data Please refer to DS document for the assay protocol.

TNF-alpha TYPICAL DATA

For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of TNF-α is less than 2.321 pg/mL.

 

验证(Validation)

样本值(Sample Values)

Serum – Samples from apparently healthy volunteers were evaluated for the presence of human TNF-α in this assay. No medical histories were available for the donors used in this study.
Cell Culture Supernatant – Human peripheral blood mononuclear cells (PBMCs) were cultured and stimulated using CD28/OKT3 antibody then detected TNF-α secretion amount.

TNF-alpha SAMPLE VALUES

稀释线性(Dilution Linearity)

High concentrations (1200pg/mL) of human TNF-α were added to different diluted substrates for gradient dilution to evaluate the linearity of the assay.

TNF-alpha DILUTION LINEARITY

批内差异(Intra-Assay Statistics)

20 replicates of each of 3 samples containing different TNF-α concentrations were tested in one assay. Acceptable criteria: CV<15%.

TNF-alpha INTRA-ASSAY STATISTICS

批间差异(Inter-Assay Statistics)

3 samples containing 3 different concentrations of TNF-α were tested in independent assays. Acceptable criteria: CV<15%.

TNF-alpha INTER-ASSAY STATISTICS

回收率(Recovery)

TNF-α was spiked into 3 human serum samples, and then analyzed. The average recovery of TNF-α for serum samples is 101.0%, range is 89.6% - 111.4%.

TNF-alpha RECOVERY

 

组分(Materials Provided)

IDComponentsSize
CRS002-C01Pre-coated Anti-TNF-α Antibody Microplate1plate
CRS002-C02Human TNF-α Standard20μg
CRS002-C03Biotin-Anti-TNF-α Antibody20μg
CRS002-C04Streptavidin-HRP50 μL
CRS002-C0510xWashing Buffer 50 mL
CRS002-C062xDilution Buffer50 mL
CRS002-C07Substrate Solution12 mL
CRS002-C08Stop Solution7 mL

 

前沿进展

 
 
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TNF-alpha靶点信息
英文全称:Tumor necrosis factor α
中文全称:肿瘤坏死因子α
种类:Homo sapiens
上市药物数量:61详情
临床药物数量:63详情
最高研发阶段:批准上市
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