膜杰作
Star Staining
分子别名（Synonym）
CD27,TNFRSF7,S152,T14,Tp55
表达区间及表达系统（Source）
Rat CD27, His Tag (CD7-R52H9) is expressed from human 293 cells (HEK293). It contains AA Thr 21- Arg 183 (Accession # NP_001019506.1).
Predicted N-terminus: Thr 21
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus
The protein has a calculated MW of 19.8 kDa. The protein migrates as 30-35 kDa and 50-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Rat CD27, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
活性（Bioactivity）-ELISA
Immobilized Mouse CD27 Ligand, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CDL-M5251) at 2 μg/mL (100 μL/well) can bind Rat CD27, His Tag (Cat. No. CD7-R52H9) with a linear range of 2-39 ng/mL (QC tested).
Protocol
Immobilized Human CD27 Ligand, Fc Tag (active trimer) (MALS verified) (Cat. No. CDL-H5266) at 2 μg/mL (100 μL/well) can bind Rat CD27, His Tag (Cat. No. CD7-R52H9) with a linear range of 2-78 ng/mL (Routinely tested).
Protocol
背景（Background）
CD Antigen CD27 is also known as Tumor necrosis factor receptor superfamily member 7 (TNFRSF7), which belongs to TNF-receptor superfamily. CD27 / TNFRSF7 is found in most T-lymphocytes. CD27 / TNFRSF7 is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-κB and MAPK8/JNK. CD27 has been shown to interact with SIVA1, TRAF2 and TRAF3.
