膜杰作
Star Staining
分子别名（Synonym）
CD86,B7-2,B70,CD28LG2,LAB72,MGC34413
表达区间及表达系统（Source）
Rat B7-2, His Tag (CD6-R52H9) is expressed from human 293 cells (HEK293). It contains AA Val 29 - Ile 250 (Accession # O35531-1).
Predicted N-terminus: Val 29
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus
The protein has a calculated MW of 27.3 kDa. The protein migrates as 40-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Rat B7-2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
活性（Bioactivity）-ELISA
Immobilized Rat CTLA-4, Fc Tag (Cat. No. CT4-R5259) at 5 μg/mL (100 μL/well) can bind Rat B7-2, His Tag (Cat. No. CD6-R52H9) with a linear range of 0.02-1.25 μg/mL (QC tested).
Protocol
Immobilized Human CTLA-4, Fc Tag (Cat. No. CT4-H5255) at 5 μg/mL (100 μL/well) can bind Rat B7-2, His Tag (Cat. No. CD6-R52H9) with a linear range of 0.01-0.625 μg/mL (Routinely tested).
Protocol
Immobilized Mouse CTLA-4, Fc Tag (Cat. No. CT4-M5256) at 5 μg/mL (100 μL/well) can bind Rat B7-2, His Tag (Cat. No. CD6-R52H9) with a linear range of 0.039-1.25 μg/mL (Routinely tested).
Protocol
背景（Background）
Cluster of Differentiation 86 (CD86) is also known as B-lymphocyte activation antigen B7-2, is a type I membrane protein that is a member of the immunoglobulin superfamily, and is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells. Additionally, B72 is expressed at low levels on monocytes and can be upregulated through interferon γ. CD86 is the ligand for two different proteins on the T cell surface: CD28 (for autoregulation and intercellular association) and CTLA-4 (for attenuation of regulation and cellular disassociation). CD86 works in tandem with CD80 to prime T cells. Recent study has revealed that B7-2 promotes the generation of a mature APC repertoire and promotes APC function and survival. Furthermore, the B7 proteins are also involved in innate immune responses by activating NF-κB-signaling pathway in macrophages. CD86 thus is regarded as a promising candidate for immune therapy. CD86+ macrophages in Hodgkin lymphoma patients are an independent marker for potential nonresponse to firstline-therapy.
