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Cynomolgus DLK1 / FA1 Protein, His Tag

分子别名(Synonym)

DLK1,FA1,Protein delta homolog 1,Pg2,Fetal antigen 1

表达区间及表达系统(Source)

Cynomolgus DLK1, His Tag (DK1-C52H7) is expressed from human 293 cells (HEK293). It contains AA Ala 24 - Cys 306 (Accession # A0A2K5TMQ6-1).

Predicted N-terminus: Ala 24

Request for sequence

蛋白结构(Molecular Characterization)

DLK1 Structure

This protein carries a polyhistidine tag at the C-terminus

The protein has a calculated MW of 32.1 kDa. The protein migrates as 36-46 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

DLK1 SDS-PAGE

Cynomolgus DLK1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

 

活性(Bioactivity)-ELISA

DLK1 ELISA

Immobilized Cynomolgus DLK1, His Tag (Cat. No. DK1-C52H7) at 1 μg/mL (100 μL/well) can bind anti DLK-1 antibody with a linear range of 0.078-5 μg/mL (QC tested).

Protocol

 

背景(Background)

Protein delta homolog 1 is a protein that in humans is encoded by the DLK1 gene. It is expressed as a transmembrane protein, but a soluble form cleaved off by ADAM17 is active in inhibiting adipogenesis, the differentiation of pre-adipocytes into adipocytes.

 

前沿进展

 
 
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DLK1靶点信息
英文全称:Protein delta homolog 1
中文全称:
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上市药物数量:0详情
临床药物数量:2详情
最高研发阶段:临床一期
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