膜杰作
Star Staining
产品概述（Product Details）
ClinMax™ Human IL-8 ELISA Kit, PRO is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant human IL-8 that is present in biological samples, such as human serum, plasma, and cell culture supernatants. Our ClinMax™ ELISA Kit provides several benefits:
- Standards to calibrate with NIBSC/WHO standards for comparable results.
- Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
- High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
- Simplified and straightforward protocols and ready-to-use reagents to save assay time.
应用说明（Application）
The kit is developed for quantitative detection of natural and recombinant human IL-8 in serum, plasma and cell culture supernatants.
It is for research use only.
流程图（Workflow）
关键信息（Key Features）
| Analyte | IL-8 |
| Assay Type | Sandwich-ELISA |
| Reactivity | Human |
| Sensitivity | 3.0 pg/mL |
| Range | 6.25 pg/mL-400 pg/mL |
| Assay Time | 1.75 hr |
| Sample Type | Cell Culture Supernatants, Plasma, Serum. |
| Sample volume | 50 uL |
| Format | 96-wells plate breakable into 12 x 8 wells strips |
Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
存储（Storage）
Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
典型数据-Typical Data Please refer to DS document for the assay protocol.
For each experiment, each ELISA plate needs to set the standard curve. The minimum detectable concentration of CEA-C004 is less than 3.0 pg/mL.
验证（Validation）
稀释线性（Dilution Linearity）
Three human serum samples with high concentrations of IL-8 were diluted 1:2, 1:4, 1:8, 1:16, 1:32 with Dilution Buffer to produce samples with values within the dynamic range and then assayed. On average, 103.12% of IL-8 was detected from serum samples.
批内差异（Intra-Assay Statistics）
Ten replicates of each of five samples containing different IL-8 concentrations were tested in one assay , Intra-Assay Precision CV＜10%.
批间差异（Inter-Assay Statistics）
Five samples containing different concentrations of IL-8 were tested in three independent assays , Inter-Assay Precision CV<15%.
回收率（Recovery）
Recombinant IL-8 was spiked into 5 human serum samples, and then analyzed. On average, 97.54% of IL-8 was recovered from serum samples.
Recombinant IL-8 was spiked into cell supernatant sample, and then analyzed. On average, 98.87% of IL-8 was recovered from serum samples.
干扰效应(Interference effect)
Bilirubin (simulated jaundice) concentration should be less than 20mg/dL, hemoglobin (simulated hemolysis) concentration should not be higher than 3500mg/dL, triglyceride (simulated lipemia) concentration may not be higher than 2.0g/L, Heparin concentration should be less than 40U/mL, EDTA concentration should be less than 4mg/mL, and Sodium citrate concentration may not be higher than 40mg/mL, it does not affect the detection result.
组分（Materials Provided）
| ID | Components | Size |
| CEA004-C01 | Pre-coated Anti-IL-8 Antibody Microplate | 1 plate |
| CEA004-C02 | IL-8 Calibrator | 20 μg×2 |
| CEA004-C03 | Biotin-Anti-IL-8 Antibody Concentrated Solution | 100 μL |
| CEA004-C04 | Biotin Antibody Dilution Buffer | 8 mL |
| CEA004-C05 | IL-8 Streptavidin-HRP Concentrated Solution | 0.5 mL |
| CEA004-C06 | Streptavidin-HRP Dilution Buffer | 15 mL |
| CEA004-C07 | 20× Washing Buffer | 50 mL |
| CEA004-C08 | Sample Dilution Buffer | 15 mL×2 |
| CEA004-C09 | Substrate Solution | 12 mL |
| CEA004-C10 | Stop Solution | 6 mL |
