膜杰作
Star Staining
分子别名（Synonym）
IL-23 alpha & IL-12 beta
表达区间及表达系统（Source）
Human IL-23A&IL-12B Heterodimer Protein, premium grade (ILB-H5219) is expressed from human 293 cells (HEK293). It contains AA Ile 23 - Ser 328 (IL-12B) & Arg 20 - Pro 189 (IL-23A) (Accession # P29460-1 (IL-12B) & Q9NPF7-1 (IL-23A)).
Predicted N-terminus: Ile 23
Human IL-23A&IL-12B Heterodimer Protein, premium grade (ILB-H5219), designed for preclinical stage, has the same activity and performance with GMP Human IL-23A&IL-12B Heterodimer Protein, which enables a seamless transition from preclinical development to clinical phases. Premium Grade product offer a cost efficient alternative of GMP Grade products for the early development phase when safety of raw materials is not top priority. By using Premium Grade products in early development phase, you can transition easily into clinical and commercial phase without need to revalidate the raw materials and modify manufacturing process.
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蛋白结构（Molecular Characterization）
This protein carries no "tag".
The protein has a calculated MW of 55.0 kDa. The protein migrates as 58 kDa±3 kDa under reducing (R) condition, and 58 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 0.1 EU per μg by the LAL method.
无菌（Sterility）
Negative
支原体（Mycoplasma）
Negative.
纯度（Purity）
>95% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 24 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Human IL-23A&IL-12B Heterodimer Protein, premium grade on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human IL-23A&IL-12B Heterodimer Protein, premium grade (Cat. No. ILB-H5219) is more than 85% and the molecular weight of this protein is around 50-70 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-Bioactivity CELL BASE
Human IL-23A&IL-12B Heterodimer Protein, premium grade (Cat. No. ILB-H5219) stimulates proliferation of Human IL-23 R/IL-12 R beta 1(Luc) HEK293 Reporter Cell. The specific activity of Human IL-23A&IL-12B Heterodimer Protein, premium grade is > 1.80 X 10^3 U/mg (QC tested).
Protocol
活性（Bioactivity）-ELISA
Immobilized Human IL-23A&IL-12B Heterodimer Protein, premium grade (Cat. No. ILB-H5219) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human IL23A&IL12B P40 domain Antibody, Human IgG1 with a linear range of 0.02-1 ng/mL (QC tested).
Protocol
Immobilized Human IL-23A&IL-12B Heterodimer Protein, premium grade (Cat. No. ILB-H5219) at 5 μg/mL (100 μL/well) can bind Human IL-12 R beta 1, Fc Tag (Cat. No. ILB-H5255) with a linear range of 0.2-3 ng/mL (Routinely tested).
Protocol
背景（Background）
Interleukin-23 subunit alpha (IL-23 alpha) can associates with IL12B to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.
